Nicanor I Moldovan, PhD, Affiliate
College of Medicine
Internal Medicine / Cardiovascular Medicine
Res Assoc Professor
Molecular Biology and Cancer Genetics
Neovascularization, Pathologic, Arteriosclerosis, Disease Models, Animal, Oxidative stress, Neoplasms, Inflammation, Atherosclerosis
a) Detection of circulating endothelial and other progenitor cells in human blood, using an original solid phase assay (called CellTrap, in development). This project is funded through an ARRA/RC2/GO stimulus grant from NIH. b) Study of plasticity of circulating progenitor cells towards the endothelial phenotype. Within another NIH funded project, we developed a novel model of neovascularization in adult animals, based on matrix degradation by migrating cells (‘tunneling’), followed by ‘cell columns’ formation and in situ differentiation of progenitor cells into ‘fibro-vascular bundles’. c) Identification of biochemical factors limiting survival of circulating progenitor cells spontaneously recruited in damaged tissues. We are using mouse models of myocardial infarction, and transgenic animals deficient in the transcription factor slug involved in epithelial-to-mesenchymal transition. The project was funded by an American Heart Association Grant in Aid. d) Biomechanical factors limiting the efficiency of cell therapy and in general the traffic of cells (including tumor cells) in the bloodstream. In this regard, we defined and now we are studying experimentally the cellular structural robustness, and developing new computational models of cell structure. We are also exploring the role of water in the control of biomechanical properties of the cytoskeleton. A subproject is being funded by an Pelotonia Undergraduate Award. e) Use of progenitor cells for tissue engineering of peri-implant space. This project, also funded by NIH and a seed grant from Institute of Materials Research at OSU proposes a different approach to deal with the ubiquitous ‘foreign body’ reaction and subsequent fibrous encapsulation affecting implanted biosensors and drug delivery devices: namely to ‘treat’ them before impanation with stem/progenitor cells for stimulation peri-implant neovascularization. As model implant we are using an oxygen sensor detectable by electronic paramagnetic resonance in live animals, materials obtained by electrospinning as scaffolds to harbor the cells, and mathematical modeling of neovascularization and to account for molecular diffusion around the implant. All these methods are either inspired from or applicable to cancer biology.