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Services

NEW SERVICE: The new BD FACSAria III Flow Cytometer.

This instrument has new laser capabilities: 488 nm, 640 nm, 561 nm and 355 nm lasers (blue, red, yellow-green and UV). The new BD FACSAria III will deliver high-speed cell-sorting and additional multicolor capabilities beyond what is currently offered in the ACSR.

The new instrument will effectively double the sorting capability of the shared resource. In addition, it will be equipped with four lasers: the usual blue 488 nm and red 640 nm our instruments currently have, along with an additional UV 355 nm and a yellow 561 nm laser, giving us the ability to analyze 12 fluorescent parameters. The 561 will give us capabilities to analyze and sort cells expressing mCherry and DsRed. The UV excitation will give better excitation for DAPI and Hoechst dyes, and include a filter set for side populations. A redesigned flow cell will enable the Aria III to sort cell cycle samples. Like our current Aria IIu within the core, the new Aria III will be capable of sorting four populations into microtubes, 12x75 mm tubes, two ways into 15 mL conical tubes, along with various plates from six to 384 wells.

Our services include:

Cell Analysis (including immunophenotyping, DNA Cell Cycle Analysis, Annexin V apoptosis, etc.)

Single cell analysis is available using the BD FACSCalibur and BD LSR II systems.

Cell Sorting

High-speed cell sorting is available using the BD FACSAria IIu and Aria IIl.

Data Analysis

Data Analysis is available for analyzing previously stored data for statistical purposes and preparing data for presentations in abstracts, grants, manuscripts or publications in scientific journals.

Instrumentation and Equipment

The ACCSSR currently hosts a wide array of cytometers and cell sorting instrumentation. Below are some comprehensive tables to help select the appropriate instrument for your experiment.

Cell sorters:

The shared resource has two cell sorters available: the Aria IIu and Aria III. The Aria IIu has two lasers, a 488 blue and 633 red and detectors for up to seven colors. The Aria III adds additional 355 UV and 561 yellow, green lasers along with additional detectors, taking it up to eleven colors. These lasers allow the Aria III to perform applications, including side populations, using Hoescht dyes, fluorescent proteins like mCherry, and cell ploidy. Both are capable of sorting up to four populations at a time, and plate sorting including, but not limited to, 96 wells.

Analyzers:

The shared resource has two analyzers, a two-laser four color FACSCalibur and a three- laser 12-color LSR II. Both instruments have 488 and 633 lasers, while the LSR II has an additional 405 violet laser. The violet laser and the accompanying additional detectors give you an additional wide variety of color options for your analysis applications.

Flurochrome guide to selecting instrumentation:

flurochrome

Assay guide to selecting instrumentation:

​Aria III

​Aria IIu

​Calibur

​LSR II

​1 Color Assay

​X

​X

​X

​X

2-4 Color Assay​

​X

​X

​X

​X

5-8 Color Assay​

​X

​X

​X

​UV Applications

​X

mCherry/RFP​

​X

Plate Sorting​

​X

​X

Multi-population sorting​

​X

​X

 

FY14 Fees

Analysis Calibur
$45.00 hour

Analysis Calibur (Mgr Assist)
$60.00 hour

Analysis LSR II
$45.00 hour

Analysis LSR II (Mgr Assist)
$60.00 hour

Analysis Aria (Mgr Assist)
$75.00 hour

Sorting Aria (Mgr Assist)
$75.00 hour

Analysis Aria III (Mgr Assist)
$75.00 hour

Sorting Aria III (Mgr Assist)
$75.00 hour

Sort Set-up Fee
$45.00 hour

Computer Analysis and/or Consultation
$75.00 hour

FACS Training – group of three
$180.00 session

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