Services

The Leukemia Tissue Bank Shared Resource (HMSR) facilitates successful translation of basic research to the clinical setting by providing an extensive repository of tissue samples from patients with leukemia, lymphoma or myeloma, accompanying pathologic, cytogenetic and clinical data for correlation of clinical and biological results, and expert training and support for cancer investigators.

An NCI-sponsored biorepository, the LTBSR has more than 40,000 vials of cryopreserved viable cells and 13,000 vials of matched frozen plasma and/or serum samples from more than 4,000 patients treated for leukemia and other hematologic malignancies.

The LTBSR’s services include:

Tumor Cell Isolation, Derivative Sample Preparation and Cryopreservation of Viable Cells

Cells from bone marrow aspirates and peripheral blood samples are isolated by Ficoll density gradient centrifugation. After a viable cell count is completed, cells are washed and allocated for derivative sample preparation as determined by the corresponding treatment, correlative studies or research protocol. Remaining viable cells are cryopreserved and stored long-term in a liquid nitrogen freezer.

Cytological Slide Preparation

The cytological centrifuge is a specialized instrument to deposit cells uniformly into a defined area on a glass slide. These slides are then preserved and stored for future microscopic evaluations (e.g., immunocytochemistry).

Dangerous Goods Shipping

Packages containing human samples and/or dry ice require special shipping procedures. The LTBSR is experienced in the proper routes to ensure safe delivery of samples to off-campus recipients.

DNA Isolation from Mononuclear Cells

To investigate genetic/epigenetic changes that may occur with disease, the LTBSR isolates high-quality DNA from fresh or cryopreserved mononuclear cell fractions of bone marrow aspirate or peripheral blood samples.

DNA Isolation from Buccal Swabs

DNA isolation from buccal (cheek lining) cells collected on a swab or in mouthwash provides normal germline DNA from our patients so that disease-specific changes can be identified.

Materials Processing for Studies

The LTBSR can process material for studies including protein, RNA and DNA, and also can prepare cDNA from RNA samples. The resource also coordinates central molecular and flow cytometry analyses for studies as needed. Central isolation of protein and nucleic acids has allowed the LTBSR to provide investigators with the exact amount of uniform, high-quality material required, thereby conserving material obtained from a single vial of cells for additional studies. We make every attempt to customize sample handling, processing and storage to suit each clinical trial we support.

PAXgene Sample Processing

In cases where immediate Ficoll separation is not feasible, the LTBSR utilizes t he PAXgene Blood system. This consists of specialized tubes for blood collection and stabilization as well as purification of high-quality DNA or RNA in a single-tube procedure.

Primary Cell Culture

Following isolation from blood or marrow, patient tumor cells can be cultured for a limited time in sterile media. This procedure is useful for testing experimental agents or conditions, allowing the researcher to monitor changes in live tumor cells under carefully controlled conditions.

Quality Control of Derivative Specimens

This includes highly sensitive analysis of DNA, RNA and protein preparations via a new Qubit fluorometer. This instrument enables quick determination of concentration and quality of isolates while preserving the bulk of the sample for research.

Research Consultation – Sample Selection and Data Processing

Our staff will work with investigators to customize sample procurement, handling, processing and storage. We work with investigators to identify samples in the biorepository to match their research requirements.

RNA Isolation from Mononuclear Cells

We prepare RNA according to a variety of methodologies to best suit requirements of the individual research project.

Sample Retrieval, Packaging and Shipping

Once a sample set has been identified for a specific clinical trial or research study, we retrieve samples from long-term storage (and, if needed, prepare derivative specimens) to be released either to investigators at Ohio State or collaborators around the world.

Serum or Plasma Isolation

Serum (fluid from clotted blood) and plasma (fluid from non-clotted blood) can be derived from whole blood as needed according to the research protocol.

Wright/Giemsa with Blast Count

The LTBSR can perform staining and microscopic evaluation of cytological slides to determine percent of tumor cells in a sample.

Instrumentation

The LTBSR operates in accordance with NCR Best Practices for sponsored repositories, and offers OSUCCC members and the cancer research community access to the following instrumentation, equipment and supplies:

Beckman Coulter ViCell Counter for cell counts and viability data on each Ficoll-separated blood or marrow sample prior to cryopreservation.

Benchtop Centrifuges for mononuclear cell separation

Biological safety Cabinets for sterile processing (and if needed, cell culture) of samples

Buccal Swab and Mouthwash Collection Kit s for the collection and extraction of normal DNA

Computer Workstations for electronic database management

Dangerous Goods Shipping Containers for sending samples to approved outside investigators

Dry Ice for on-campus transport or shipping

MVE Automated Filling System for efficient liquid nitrogen (LN2) use. The system includes a service area and automatic tank switcher where up to four LN2 tanks can be stationed. Vacuum-jacketed piping leads to filling stations for each LN2 freezer.

MVE LN2 Freezers for safe long-term sample storage without direct contact with LN2. Freezers hold 15,500-36,400 samples, depending on rack size and storage box profile.

NanoDrop Spectrophotometer for spectrophotometric analysis of absorbance ratios to quantify derivative specimens. This spectrophotometer quickly determines the concentration of RNA, DNA or protein, and uses an extremely small sample volume. This enables the LTBSR to perform appropriate quality checks of samples while preserving the bulk of the sample for research.

Polymerase Chain Reaction Thermocycler for quality control of procured and prepared materials

Rees Scientific Environmental Monitoring System for 24/7 monitoring of all LTBSR freezers and refrigerators. The Rees system provides an hourly readout of monitored units and immediate telecommunication with individuals in the event of temperature fluctuation beyond the specified range.

Refrigerated Microcentrifuges for derivative sample preparation

QIAcube™ Biorobot for automated sample preparation for RNA, DNA and protein using Qiagen® spin-column technology. The QIAcube automates simultaneous  sample preparation for more consistent sample products and improved lab efficiency.

Qubit Fluorometer for highly sensitive determination of the concentration and quality of derivative specimens while preserving the bulk of the sample for research.

Forms

Preliminary Sample Request Form

Application for Materials/Services Form

Fees

Buccal swab DNA collection kit – CCC Member
$12.00 sample

Dangerous goods shipping charge – CCC Member
$50.00 supply

Dangerous goods shipping container – CCC Member
$30.00 supply

DNA isolation from buccal swab – CCC Member
$35.00 supply

Dry ice (for transport/shipping) – CCC Member
$30.00 sample

Positive selection/depletion (1st sample)
$130.00 sample

Positive selection/depletion (each add. 10 samples)
$100.00 sample

Preparation of cytological slides –- CCC Member
$12.50 sample

Procure w/RNA or DNA lysis prep only – CCC Member
$75.00 sample

Sample prep-lymph node/small tissue biopsy – CCC Member
$105.00 sample

Sample retrieval (per 10 samples) – CCC Member
$1.00 sample

Search charge – Bucci – CCC Member
$65.00 hour

Search charge – Faculty – CCC Member
$300.00 hour

Serum/plasma prep w/cryopres – CCC Member
$12.00 sample

Standard PBMC procure w/cryopres and LN2 (<10vials)
$105.00 sample

Storage Fee
$10.00 rack

Technician time – CCC Member
$35.00 hour

Wright/Giemsa with blast count – CCC Member
$15.00 sample

DNA isolation (thawing cells required)
$30.00 sample

Thaw viable cells for RNA or DNA isolation
$75.00 sample

BD Cell Prep Tube (mononuclear cells)
$85.59 sample

RNA isolation – Qiagen (thawing cells required)
$9.20 sample

RNA isolation –  Trizol (thawing cells required)
$9.20 sample

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