Proteomics


Director: Michael Freitas, PhD
Senior Faculty Advisor: Vickie Wysocki, PhD
Technical Director: Liwen Zhang, PhD

The Proteomics Shared Resource (PSR) provides OSUCCC investigators access to advanced mass spectrometry instrumentation and analysis for protein identification, characterization, and quantification. Using a variety of analytical platforms, researchers are able to discover novel differentially expressed proteins in serum, urine, BAL fluid, saliva, frozen tissues, formalin-fixed tissues and cell lysates. The PSR is part of the Campus Chemical Instrument Center managed by the OSU Office of Research, with significant operational management by the OSUCCC. The PSR is located within OSUCCC space in the Biomedical Research Tower. The Proteomics Shared Resource (PSR) is an interdisciplinary unit that provides cancer researchers with technical expertise and state-of-the-art instrumentation needed to identify proteins, protein modifications, protein interactions and protein biomarkers as well as protein quantitation studies in cancer samples. The PSR can identify proteins from solution, 1D and 2D gels using electrophoresis and imaging equipment, robotic sample handlers and (tandem) mass spectrometers.   

Proteomic Shared Resource Goals 

  • Provide a high-quality, affordable service for identifying proteins, protein modifications, protein interactions and protein biomarkers as well as protein quantitation studies
  • Provide consultative services and technical assistance in experimental design and implementation in protein sample preparation, separation and purification, as well as in image analysis, mass spectrometry and data mining
  • Provide education and training for basic and clinical investigators in proteomics analysis applications and capabilities
  • Provide advanced methods of sample pre-fractionation prior to mass spectrometric analysis and protein identification
  • Provide for the analysis of complex mixtures of proteins by 2D-HPLC and tandem mass spectrometry, especially for evaluating protein and chemical biomarkers for cancer and therapeutic regimens

Expertise

Director: Michael Freitas, PhD
Senior Faculty Advisor: Vickie Wysocki, PhD
Technical Director: Liwen Zhang, PhD
Associate Director of CCIC MSP: Arpad Somogyi, PhD
Senior Research Associate: Nan Kleinholz
Research Associate: Cindy James, PhD
Research Associate: Jeremy Keirsey, MS
Research Assistant: Yu Cao, PhD
Systems Administrator: Andy Hall
Administrator: Robin Gates

Michael Freitas, PhD, is associate professor in the Department of Molecular Virology, Immunology and Medical Genetics at The Ohio State University. Having worked in the field of mass spectrometry and proteomics for 22 years, he has spent the last 14 years applying mass spectrometry to the analysis of histone modifications. Dr. Freitas has published 85 papers in peer reviewed journals with >1,400 citations and an H-index of 29. He performed his postdoctoral research at the National High Magnetic Field laboratory under the direction of Dr. Alan G. Marshall and his graduate work at Kansas State University under the direction of Dr. Richard A. J. O’Hair. During this time, his research focused on high-resolution, high-mass accuracy Fourier Transform Ion Cyclotron Resonance (FT-ICR) MS and biomolecule characterization. Dr. Freitas is the Director of the Proteomics Core for Ohio State’s Leukemia SCOR and scientific advisor to Ohio State’s Mass Spectrometry and Proteomics Facility. 

Vicki Wysocki, PhD, is a professor in the Department Chemistry and Biochemistry at Ohio State and Ohio Eminent Scholar of Macromolecular Structure and Function. She is also Director of the Campus Chemical Instrument Center at Ohio State, which provides NMR, X-ray and Mass Spectrometry and Proteomics services. Wysocki has a demonstrated record of successful and productive research projects, both as PI and co-investigator. Recently, she has pioneered the use of surface-induced dissociation tandem mass spectrometry coupled to ion mobility spectrometry for the characterization of large non-covalent protein and nucleoprotein complexes. For more than 20 years, Wysocki’s group has been involved in defining peptide fragmentation mechanisms and  currently is involved in applied proteomics research, including discovery of microorganism protein biomarkers in human biofluids and discovery of markers of disease.

Liwen Zhang, PhD, is a research scientist who specializes in post-translational modification and protein quantitation analyses, while also providing technical expertise on mass spectrometry and proteomics. She will serve as Technical Director for the proposed projects. Dr. Zhang has worked extensively in detecting post-translational modifications, crosslink or amino acid mutation of proteins. In addition, she works on label-free or isotope-labeling protein quantitation using mass spectrometry coupled with chromatography. Dr. Zhang earned her doctorate at The Ohio State University, where she studied histone modifications using mass spectrometry. A member of PSR for 11 years, she has developed good relationships with diversified collaborative projects and co-authored more than 30 scientific publications.

Arpad Somogyi, PhD, Associate Director of CCIC MS&P, has 30+ years of experience in mass spectrometry and 18 years in MS Facility Management. Dr. Somogyi's is the Associate Director of the OSU Campus Chemical Instrument Center (CCIC). For the PSR, Dr. Somogyi assists Dr. Liwen Zhang in the development of new initiatives such as overseeing installation of the new instruments, building metabolomics services, supervising administrative staff in billing and web-site maintenance, and overseeing difficult instrument troubleshooting issues.  Dr. Somogyi’s role is principally as advisor.

Nan Kleinholz is not only a MALDI-TOF technical expert, but also an expert in chromatography. This is especially valuable for liquid chromatography-mass spectrometry (LC-MS/MS) studies relevant to proteomics in which there are complex mixtures, and the mass spectrometry resolution is only as good as the initial LC separation. Kleinholz also helps researchers develop chromatographic methods for 2D-capillary LC/MS/MS.

Cindy James, PhD, has been active in research involving protein purification and characterization throughout her career. Through her expertise in protein purification, 2D polyacrylamide gel separations and subsequent image analysis of the gels, Dr. James helps researchers develop sample fractionation and extraction for improved gel electrophoresis methods to separate important components prior to mass spectrometric analysis. Dr, James also is able to assist researchers with both 1D and 2D Western blot analysis..  She earned her PhD from The University of Akron, Ohio.

Jeremy Keirsey, MS, has a varied research background that includes contributions in the fields of DNA repair and cancer biology. Keirsey is extensively involved in sample preparation and processing of proteomic, lipidomic and metabolomic samples, as well as in conducting quantitative proteomics experiments. He has extensive experience performing Difference Gel Electrophoresis (DIGE) to discover protein expression and/or protein phosphorylation changes from a variety of sample sources. In addition to supporting the PSR by processing proteomic samples on our LTQ mass spectrometer and DSQ II GC-MS, Keirsey  assists in maintenance of our DSQ II GC-MS and performs GC-MS experiments.

Yu Cao, PhD, designs and conducts experiments and research for projects submitted to the PSR with expertise in qualitative, semi-quantitative and quantitative metabolomics. Yu develops new sample preparation techniques, chromatographic (HPLC, nanospray) protocols and mass spectrometry methods. In addition to performing bioinformatics and/or quantitative calculations and database searches as necessary, Yu trains self-operation users. In charge of the operation and routine maintenance of the triple quadruple mass spectrometer, Yu has been with the PSF since 2013. 

Andy Hall is responsible for all network and bioinformatic hardware. Bioinformatics is a critical aspect of high-throughput proteomics; in some instances, the huge search files can take days to search. Moving, disseminating and storing this data is critical to this resource’s operation and publication abilities, making Hall’s work indispensable to the PSR. 

Robin Gates, the administrator for the facility, assists the Associate Director in the day-to-day operations of the facility, and assists the director of CCIC when needed. As administrator, Gates purchases all supplies and equipment, maintains all usage records, processes monthly billing for internal and external users and maintains all revenue and expenditure records. Gates has combined administrative experience of 27 years. She has been with the facility since 1999. 

Cross-Institutional Work 

  • University of Akron
  • University of Alaska
  • Battelle Memorial Institute
  • Bowling Green State University
  • University of California at Los Angeles (UCLA)
  • Case Western Reserve University
  • Cincinnati Children’s Hospital Medical Center
  • University of Cincinnati
  • Dental Stem Cell Development
  • Indiana University
  • Miami University (Ohio)
  • MRI Global Research Institute
  • Northeastern Ohio Medical University
  • Northern Illinois University
  • Ohio University (Athens)
  • University of Pittsburgh
  • Proctor and Gamble Co.
  • Purdue University
  • The Research Institute at Nationwide Children’s Hospital
  • Thomas Jefferson University
  • University of Toledo
  • Virginia Commonwealth University
  • University of Virginia
  • College of Wooster
  • Wright State University
  • Yale University
  • Youngstown State University 

Contact Us

Proteomics Shared Resource
Biomedical Research Tower
460 W. 12th Ave.
Room 250
Columbus, OH 43210

Ph: 614-688-0521
Fax: 614-292-5955 

Availability: Monday – Friday
Core Hours: 7 a.m. – 5 p.m. 

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