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Services

The Small Animal Imaging Center Shared Resource (SAISR) is a state-of-the-art, small animal imaging facility available to investigators at OSUCCC–James as well as to other academic and commercial institutions. 

Services include:

NEW SERVICE: Magnetic resonance microscopy (MRM) for evaluating phenotypic differences in embryos of genetically engineered mice. This technique can be used to obtain an isotropic 3D MR image of an embryo at a spatial resolution on the order of 30-40 microns. MRM can be performed on the sample prior to histologic staining of the sample and will complement the pathology review provided by the Comparative Pathology and Mouse Phenotyping Shared Resource.

High-Resolution Image Data Acquisition 

The SAISR provides high-resolution anatomical and functional imaging of rodent models of disease and ex vivo specimens. Technicians and imaging scientists trained in the use of the specialized image acquisition equipment will collect high-quality image data for individual investigators. In-house training of an investigator’s laboratory personnel in use of the imaging equipment (ultrasound and BLI/FL) is also available to those groups who demonstrate a specific need for extensive imaging studies.

Data Interpretation

The SAISR experts help investigators interpret images obtained from the facility’s instrumentation. Our associates are experienced in distinguishing normal and abnormal features in in vivo images of animals, and have the training and background to differentiate imaging artifacts from real results. Investigators are encouraged to review their results and data interpretation with SAISR personnel upon imaging study completion.

Image Post-Processing and Quantitative Image Analysis 

The SAISR offers quantitative image analysis, and each imaging instrument has modality-dependent image analysis software. Additional image analysis and data interpretation is provided via open-source image analysis software and custom-designed software developed by the SAISR and OSC.

Consultation on Design Imaging Studies

The SAISR’s imaging scientists can help design imaging studies for each investigator’s specific research applications. We recommend contacting a SAISR representative prior to setting up an imaging study to determine the most appropriate imaging modality and protocol for each imaging study.

Training and Education

The SAISR provides hands-on training for individual imaging modalities and workshops on image acquisition and data analysis. We also hold seminars on specific research topics in small animal imaging.  

Instrumentation Equipment

The Small Animal Imaging Center Shared Resource (SAISR) facility houses high-resolution small animal imaging instrumentation and offers assistance from experts in: operating each imaging modality, small animal handling procedures and analytical software support for quantitative image analysis.

The following micro-imaging equipment includes magnetic resonance imaging (MRI), positron emission tomography (PET), single-photon emission computed tomography (SPECT), X-ray computed tomography (X-ray CT), high-frequency ultrasound (US), bioluminescence and fluorescence optical Imaging system (BLI/FL).

All imaging instrumentation comes fully equipped with biological (ECG, respiration, temperature) monitoring equipment and isoflurane anesthesia systems. The instrumentation can be used for in vivo animal imaging or ex vivo specimen investigations.

The AVANCE™500 WB NMR Spectrometer and Imaging System is an 11.7T vertical bore magnet that operates at 500 MHz and runs ParaVision 5.1 software. The system maintains ultra-shielding technology, which provides maximum field homogeneity, field stability and stray field reduction. The 8.9 cm diameter bore is best suited for whole-body mouse imaging.

Equipped with three RF channels (proton NMR, deuterium and broadband) and preamplifiers, the AVANCE™500 has two sets of gradient coils, MicroMouse in vivo imaging probes, BioTrig monitoring units and an in vivo cardiac gating system. MRI applications include:

  • Skeletal and soft tissue abnormalities in embryonic and adult specimens
  • DCEMRI for tumor/tissue vascularization and permeability
  • In vivo spectroscopy to measure metabolites (1H, 31P)
  • Cardiac imaging for assessing cardiac function, ischemia and infarcts 

The BioSpec 94/30 Imaging System is a 9.4T horizontal bore magnet that operates at 400 MHz and runs ParaVision™ 5.1 software. This microMRI system is designed for high-resolution MR spectroscopy and imaging of in vivo or ex vivo specimens.

The 30 cm diameter bore and extra-long table offers imaging capabilities of 1H, 13C, 19F, 23 Na, 31P on mice, rats, small rabbits and ex vivo specimens. The BioSpec also includes a BioTrig monitoring unit and in vivo cardiac gating.

MRI applications include:

  • Skeletal and soft tissue abnormalities
  • DCEMRI for tumor/tissue vascularization and permeability
  • In vivo spectroscopy to measure metabolites (e.g. 31P)
  • In vivo localized spectroscopy to measure metabolites (1H)
  • Cardiac imaging for assessing cardiac function, ischemia and infarcts
  • T1, T2, and Diffusion maps

The Vevo 2100 High-Frequency Ultrasound System is capable of 2D and 3D in vivo imaging in small animals and has a 30-micron spatial resolution and frame rates of up to 1000 fps. The system includes an 18, 24, 38 and 55 MHz transducer, a needle-guided injection apparatus, and is capable of color, pulsed wave and power Doppler, 3D imaging and advanced image analysis.

The Vevo can be used for:

  • Image-guided injections
  • Evaluating developmental biology – mouse and rat embryos
  • Calculating tumor size and blood flow
  • Quantitative evaluation of cardiac physiological function and development  

A CaliperTM IVIS Lumina II Bioluminescent Optical Imaging System is suitable for 2D in vivo and ex vivo bioluminescence and fluorescence imaging. The system is equipped with four standard emission filters ICG, Cy5.5, DsRed, GFP (810-875, 695-770, 575-650, 515-575 nm) for fluorescence imaging. 

IVIS Lumina II applications include:

  • A powerful labeling technique through gene expression results in production of luciferase
  • Provides analysis tools for quantification
  • Provides in vivo fluorescence imaging (preferable near-infrared)
  • Provides ex vivo and in vitro BLI and FL imaging

Fees

IVIS Lumina II – user-operated
$60.00 Hour/Ohio State user
$120.00 Hour/Non-Ohio State user

IVIS Lumina II – core-assisted
$105.00 Hour / Ohio State user
$165.00 Hour / Non-Ohio State user

IVIS Training
$75.00 Hour/Ohio State user
$120.00 Hour/Non-Ohio State user

Vevo 2100 – user-operated
$55.00 Hour/Ohio State user
$120.00 Hour/Non-Ohio State user

Vevo 2100 – core-assisted
$100.00 Hour/Ohio State user
$165.00 Hour/Non-Ohio State user 

Vevo 2100 Training
$75.00 Hour/Ohio State user
$120.00 Hour/Non-Ohio State user 

9.4 T MRI
$135.00 Hour/Ohio State user
$250.00 Hour/Non-Ohio State user

11.7 T MRI
$135.00 Hour/Ohio State user
$250.00 Hour/Non-Ohio State user

Data Analysis
$75.00 Hour/Ohio State user
$75.00 Hour/Non-Ohio State user 

Supplies
Market Price/Ohio State user
Market Price/Non-Ohio State user

Luciferase
Market Price/Ohio State user
Market Price/Non-Ohio State user

Data key replacement
$5,000.00/Ohio State user
$5,000.00/Non-Ohio State user

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