The ACCSSR currently hosts a wide array of cytometers and cell sorting
instrumentation. Below are some comprehensive tables to help select the
appropriate instrument for your experiment.
Instrumentation, Lasers and Location:
|
|
Description
|
Location
|
|
BD FACS Aria III
|
12 color, 4 laser (355,
488, 561 and 635nm) cell sort
|
1063 Biomedical Research
Tower
|
|
BD FACS Aria IIu
|
7 color, 2 laser (488 and
633 nm) cell sorter
|
1063 Biomedical Research
Tower
|
|
BD FACS LSR II
|
13-color, 3 laser (405nm,
488nm, and 635nm), analytical cytometer
|
1063 Biomedical Research
Tower
|
|
BD FACS Calibur
|
4 color, 2 laser (488 and
635 nm), analytical cytometer
|
1063 Biomedical Research
Tower
|
Flurochrome guide to selecting
Instrumentation:
|
|
Excitation
|
Emission
|
Aria III
|
Aria IIu
|
LSR II
|
Calibur
|
|
Hoescht 33258
|
355
|
455
|
X
|
|
|
|
|
DAPI
|
325/360/405/407
|
359
|
X
|
|
X
|
|
|
Pacific Blue
|
355/405
|
455
|
X
|
|
X
|
|
|
Alexa Fluor 405
|
355/405
|
421
|
X
|
|
X
|
|
|
Alexa Fluor 488
|
488
|
519
|
X
|
X
|
X
|
X
|
|
FITC/GFP/YFP
|
488
|
519
|
X
|
X
|
X
|
X
|
|
mCherry/RFP
|
561
|
610
|
X
|
|
|
|
|
R-PE
|
488/561
|
578
|
X
|
X
|
X
|
X
|
|
PE-Texas Red
|
488/561
|
615
|
X
|
X
|
X
|
X
|
|
APC
|
633/635
|
660
|
X
|
X
|
X
|
X
|
|
7AAD
|
488
|
647
|
X
|
X
|
X
|
X
|
|
Propidium Iodide
|
488
|
620
|
X
|
X
|
X
|
X
|
|
Alexa Fluor 647
|
633/635
|
668
|
X
|
X
|
X
|
X
|
|
PE-Cy5
|
488/561
|
667
|
X
|
X
|
X
|
X
|
|
PerCP-Cy5.5
|
488
|
695
|
X
|
X
|
X
|
X
|
|
Alexa Fluor 700
|
633/635
|
719
|
X
|
X
|
X
|
X
|
|
PE-Cy7
|
488/561
|
785
|
X
|
X
|
X
|
X
|
|
APC-Cy7
|
595/633/635/647
|
785
|
X
|
X
|
X
|
|
Assay guide to
selection Instrumentation:
|
|
Aria III
|
Aria IIu
|
Calibur
|
LSRII
|
|
1 Color Assay
|
X
|
X
|
X
|
X
|
|
2-4 Color Assay
|
X
|
X
|
X
|
X
|
|
5-8 Color Assay
|
X
|
X
|
|
X
|
|
Population Analysis
|
X
|
X
|
X
|
X
|
|
Cell Sorting
|
X
|
X
|
|
|
|
UV applications
|
X
|
|
|
|
|
mCherry/RFP
|
X
|
|
|
|
|
Plate Sorting
|
X
|
X
|
|
|
|
Multi-Population Sorting
|
X
|
X
|
|
|
Data Analysis
In addition to the instrument computers, the
ACCSSR maintains data analysis computers for users to analyze their data using,
Cell Quest Pro, DIVA, FlowJo, WinList,
or Modfit software.
Free Software
The Analytical Cytometry and Cell Sorting Resource
Lab offers one copy of FlowJo free to each lab.
Additionally, a copy is stored on each analysis computer located in the lab. To
receive a free copy, contact Bryan Mc Elwain at
614-292-FLOW (3569), or Bryan.McElwain@osumc.edu, for further information.
WinMDI is also available as a free download from The Scripps
Research Institute Flow Cytometry Core web site. WinMDI
runs on PCs and can read data files produced by Flow Cytometry Services
instruments.
Analyzing and Converting
Data from Instrumentation
Traditionally, the Flow Cytometry Services staff does not analyze
samples nor convert data to PowerPoint for users. A workstation is available
for users to utilize in this capacity. A Flow Cytometry Services operator will
gladly demonstrate how to perform this task.
For assistance with analysis or transfer, contact
a staff member to schedule an appointment.>
Usage Policy
To begin usage, an eRequest and
a New Core Lab Access Form must be completed. External Billing applies to
non-OSU.
If you have questions regarding which billing form
to use, please call 614-292-FLOW (3569)
Users who intend to use equipment by themselves
should attend the Flow Cytometry Training course held as needed for up to 3
users at a time for approximately 2.5 hrs. The Course provides essential
background in flow cytometry principles and Flow
Cytometry Services policies, and it provides enough basic instrument
training to initiate usage. Please contact the lab for more information:
Bryan McElwain
614-292-FLOW (3569)
Bryan.McElwain@osumc.edu
Because the ACCSSR staff time and availability can
be limited, please provide staff with advance notice of which flourochromes are used and what the experiment will entail.
For proper instrument set-up, users should bring
the appropriate controls and experimental samples. These usually include an
unstained control, isotype controls, single-stain
controls and combination controls. If the user is unsure of the proper
controls, please contact the ACCSSR staff, who can assist first-time users with
identifying proper controls during experimental design discussions. Please note
that without proper controls, data may not be reliable or reproducibleLaboratory Location:
|
1055/1063 Biomedical Research Tower
460 West 12th
Ave.
Columbus, Ohio 43210
Phone:
614-292-FLOW
|
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