The goal of the BBR Tissue Bank is to facilitate biomedical research using tissues at The Ohio State University.
The following services are currently offered to support this goal:
Formalin-fixed paraffin-embedded tissue: Formalin Fixed Paraffin Embedded. Tissue is fixed in formalin and then dehydrated. Tissue is then embedded in paraffin. Block can be cut to make slides.
Tissue comes the lab from the OR sterile. The lab uses methods “as clean as possible” to procure the tissue. The tissue is frozen in liquid nitrogen and then stored at -80 until transferred to the BBR lab at Polaris. The sample is frozen long term in a liquid nitrogen freezer.
Frozen OCT (Optimal Cutting Temperature - compound): Fresh Tissue is placed into molds containing OCT compound (liquid) which is frozen at -80 to form hardened blocks. – the preparation of the sample is much more rapid than with traditional histology technique (around 10 minutes vs. 16 hours). OCT can be cut to make slides without further embedding. The final slides can be used for in situ hybridization, etc. This is a method for saving tissue for future usage.
Frozen RNA Later
: RNAlater® solution is an aqueous, nontoxic tissue storage reagent that rapidly permeates tissues to stabilize and protect cellular RNA. RNAlater® solution eliminates the need to immediately process tissue samples or to freeze samples in liquid nitrogen for later processing. Tissue pieces can be harvested and submerged in RNAlater® solution for storage without jeopardizing the quality or quantity of RNA obtained after subsequent RNA isolation.
Banked frozen serum/plasma specimen:
Investigator is provided banked frozen serum or plasma specimen.
Unstained frozen section: Investigator is provided an unstained slide of sectioned frozen tissue specimen. See pricing list for charges.
H&E-stained paraffin section: Investigator is provided an H&E-stained slide of sectioned paraffin tissue specimen. See pricing list for charges.
Unstained paraffin section: Investigator is provided an unstained slide of sectioned paraffin tissue specimen. See pricing list for charges.
Fresh tissue specimen: Currently distributed through the Comprehensive Cancer Center Tissue Procurement Shared Resource.
CCC Members application
Samples are obtained through the BBR Bank while any data is received through the Information Warehouse. The Information Warehouse will be able to link the specimen sample via the BSN listed on the sample to Identifiable or de-identifiable data depending upon your IRB Approval.
Quality Control of Slides: A pathologist performs review of H&E-stained slide(s) from a surgical case. No extra charge if provided with tissue specimen.