With the OLYMPUS FV1000 MULTIPHOTON MICROSCOPE,
two-photon excitation provides optical sectioning with minimal photobleaching and phototosicity above and below the plane of focus. Because two-photon excitation occurs only at the focal plane, bleaching is only confined to this area of the secimen. Consequently, this technique can be less phototoxic to live specimens.
Routine services include:
Transmission electron microscopy of tissue thin sections
Tissues and cultured cells are embedded in epoxy resin, sectioned and stained. On the transmission electron microscope, digital micrographs are captured of the cells examined.
Transmission electron microscopy of negatively stained nanostructures
DNA, liposomes or nanostructures are mixed with heavy metal solutions and spread on a thin film. The material is observed surrounded by the dark heavy metal solution.
Scanning electron microscopy of cell cultures and biofilms
Surfaces of cultured cells, biofilms, extra cellular matrix, and nanostructures are examined at half a million times magnification.
Confocal microscopy of fixed cultures and tissue section
Fixed cells with fluorescent proteins and antibody labeled proteins is examined in thin optical sections revealing detail not seen with standard fluorescent microscopy.
Wide field fluorescent and bright field microscopy of tissues
Standard fluorescent microscopy with images captured on a CCD camera.
Training for individuals and/or groups on any of the services
MSR offers extensive training for the routine services listed here. This includes a range of options depending upon the need of the individual investigator.
Unique services offered by the MSR:
Cryo-TEM of hydrated nanostructures
Examination of hydrated nanostructures is an essential method in evaluating the loading of these important drug delivery devices. The MSR is one of only a few facilities in the country to offer this service.
Whole animal confocal microscopy with a multiphoton microscope
Following cell movement or distribution of drugs in living animals is crucial to understanding tumor growth and treatment. A new multiphoton microscope can view these activities 1 mm into a tissue of a living animal.
Live cell confocal microscopy of cultures for GFP protein movement
Cellular responses to stimuli or drugs occur in a fraction of a second. The live cell confocal can document these events at 200 frames per second.
High speed confocal microscopy with calcium sensitive dyes
Live cell confocal microscope follows calcium sensitive dye during cellular events demonstrating cell-cell signaling.
Sectioning and transmission electron microscopy of polymers
Biopolymers used as substrates for cell growth are very difficult to section for viewing in the transmission electron microscope. A cryo-ultramicrotome is used to section these soft materials.
Cryo-ultramicrotomy of small tissues for light and electron microscopy
Tissue sections thin enough to view in the electron microscope are prepared on the cryo-ultramicrotome.
Spectral separation of autofluorescence in wide field fluorescence microscopy
With a spectral CCD camera, fluorescent microscopic images are evaluated to separate overlapping colors.
Electron microscopic immunocytochemistry
Specific proteins in cells can be view with the transmission electron microscope allowing high resolution localization. Antibodies labeled with gold or silver are used.