to Ohio State’s Comprehensive Cancer Center – James Cancer Hospital and
Solove Research Institute Shared Resources Quarterly E-Newsletter. Our
goal is to provide you with important updates on our shared resources,
such as new equipment and capabilities, to highlight the excellent
science resulting from each Shared Resource, and to keep you updated on
how we provide these services. We appreciate your feedback at firstname.lastname@example.org.
NEW RATES AND FEES FOR SHARED RESOURCES!
In anticipation of a new fiscal year, we have recently completed a full cost analysis for each of our Shared Resources (SR). What does this mean? It
means that we have thoroughly gone through every service provided by
our SRs, and have compiled the actual cost to provide each respective
service. As we begin the new fiscal year on July 1, 2013, please note
that many of the prices within each SR have changed. While some did
increase, some also decreased. If you have any concerns, questions, or
requests concerning this, please contact Veronica
Rice,email@example.com. Visit the SR landing page for fee information.
the name Shared Resource implies, our facilities are set up so that
users of each SR are essentially sharing the expenses associated with
the respective service. By sharing these costs, each principle
investigator (PI) does not have to duplicate the equipment, expertise or
space. It also means that the cost passed along to each user must be
treated on a same fee for same service basis – thereby charging each
user of a facility an equitable amount. We abide by this, as this is a
federal regulation. Please note that because of this, we cannot provide
cores will be coming on board in eRamp very soon. Look for the CCTS
Clinical Research Center (CRC) and Mass Spec & Proteomics (MS&P)
in the near future. Anyone requiring training or with questions on how
to use the system, please contact Heather Richards at firstname.lastname@example.org or visit eramp.osumc.edu.
Over the next several months, our IT team will be working on an eRamp upgrade. Please stay tuned for further information!
in August, we will be deploying a new PI Publications service. This
will allow PIs to access their pubs in a web-based format and verify
that all pertinent information including PMCID is captured. It will also
allow them to attribute usage from a SR as contributing to their
Medicinal Chemistry Shared Resource (MCSR)
Chemistry has added a new staff member to help decrease turnaround
times. The OSUCCC – James and College of Pharmacy have each contributed
funding in order to bring on board the expertise of Michael Darby, PhD.
Small Animal Imaging Shared Resource (SAISR)
In Vivo Bioluminescent Imaging now available in BRT!
IVIS Lumina II from Perkin Elmer (previously Caliperl Life Sciences) is
now currently available in the Small Animal Imaging Core Facility
located in the basement of the BRT. This system is an optical imaging
system that can be used for both in vivo bioluminescent and fluorescent
imaging. The system includes a highly sensitive CCD camera, light-tight
imaging chamber and complete automation and analysis capabilities.
Information on access to and training on this instrument can be obtained
from Kim Powell, Kimerly.Powell@osumc.edu, or Anna Bratasz, Anna.Bratasz@osumc.edu.
Mass Spec&Proteomics (MS&P)
has acquired a triple quadrupole mass spectrometer. Proteomics and
Metabolomics have two distinct experimental pathways, discovery and
targeted. Previously, the instrumentation available in the MS&P was
all discovery-based by mass spectrometer, in order to perform targeted
analysis a lab must use a triple quadrupole mass spectrometer.
Previously there was no existing triple quadrupole or related instrument
in the CCIC MS&P lab that could be used to perform quantitative
proteomics and metabolomics in a high throughput manner, and therefore
could only be used for protein discovery. To qualify and validate these
proteins in large numbers of samples, a triple quadrupole mass
spectrometer was acquired. This instrument allows for highly selective
and sensitive quantitative measurements through specialized scan modes
unavailable on other instruments in the core (selected reaction
monitoring, multiple reaction monitoring as well as linked precursor,
product, and neutral loss scans).
Nucleic Acid Shared Resource (NASR)
The new HiSeq 2500 has arrived in our NASR-Illumina core. For information, please contact Pearlly Yan, PhD, 688-8462 or email email@example.com.
Improved Turnaround Times for Microarray Services
process improvements have led to shorter turnaround times for
microarray services provided by the OSUCCC – James’ NASR – Microarray
Shared Resource, a multifunctional resource that leverages the research
expertise of its staff with state-of-the-art genomics instrumentation to
provide researchers with cost-effective, high-quality data. The
facility lab specializes in gene expression with the capability for
whole genome, whole transcriptome, exome and miR expression profiling.
Current expertise includes library preparation and next generation
sequencing, commercial microarrays and custom-built microRNA arrays. For
more information about this shared resource or to discuss your upcoming
research, contact Sarah Warner, lab manager, at 292-2442, Pearlly Yan,
PhD, 688-8462 (sequencing) or Hansjuerg Alder, PhD, 292-6047 (gene
Real Time PCR Update
have a new staff member in the NASR – RTPCR. Hopefully our turnaround
times will improve.
A new QuantStudio™ 12K Flex system adds extra
capacity to the NASR’s three Applied Biosystems 7900HT Fast Real-Time
PCR Systems and its two Applied Biosystems StepOnePlus Real-Time PCR
Systems. Extra capacity will be reflected in the OpenArray format for
effortless scaling from 1 to 12,000 data points in a single run and the
simple OpenArray workflow including the Accufill System. For more
information, please contact Hansjuerg Alder, PhD, 292-6047.
nCounter System (NanoString Technologies) new single-cell expression
assay provides ultra-sensitive, reproducible and highly multiplexed
gene-expression profiling from single cells or with as little as 10pg of
total RNA. The assay linearly amplifies up to 800 target transcripts
from a single cell in a single tube without bias. For more information,
please contact Hansjuerg Alder, PhD, 292-6047.
Space, Operations and Projects (CCCLabs)
Space, Operations & Projects team has a new website. This site will
be updated as needed, but you will already find a lot of useful
- Building Access (requirements, the process involved, forms…)
including Newsletter archives with a topic index. These can be very
helpful especially as it concerns safety and EHS reminders.
policies for shared equipment, equipment disposal and transfer forms,
and the shared equipment list — broken down by type, building, and floor
- Polaris shuttle schedules and necessary forms
- Building Issues (how to report issues or concerns and improve response times)
Coming soon: contact lists, safety and training sections.
There are a couple of ways to access the site.
- Click this link to go directly to webpage.
- Visit http://cancer.osu.edu
and select Researchers and Health Care Professionals, Laboratories
& Facilities, and select Space, Operations and Projects (CCCLabs)
Small Animal Imaging (SAISR) –
addition to adding the new IVIS bioluminescence machine, SIASR will be
demoing an Olympus Bioluminescence Microscope that complements the
IVIS. For details, please contact Dr. Kim Powell at Kimerly.firstname.lastname@example.org.
Microscopy (CMIF) will be hosting a demo of Super Resolution Confocal Microscope coming later this summer! Please contact email@example.com; firstname.lastname@example.org, or Montione.email@example.com for further information.
Analytical Cytometry/Flow (ACSR)–
Cytometry/Flow (ACSR) will be hosting a two day training session,
hosted by external experts. For more information, please contact firstname.lastname@example.org.
Change for Mass Spectrometry and Proteomics (MS&P)-
Mass Spectrometry and Proteomics Facility (MS&P) has acquired a
Waters Xevo-TQ-S triple quadruple mass spectrometer complete with a
nano-Acquity 2d UPLC system. Proteomics and Metabolomics have two
distinct experimental pathways for quantization of biomolecules,
discovery and targeted. Historically, the instrumentation available in
the MS&P was all discovery based. Targeted metabolomic and
proteomic analysis utilizes a triple quadrupole mass spectrometer.
Previously there was no existing triple quadrupole in the MS&P lab
that could be used to perform targeted quantitative proteomics and
metabolomics in a high throughput manner. Now the MSP can perform both
discovery based and targeted based proteomics and metabolomics. After a
biomolecule of interest is discovered, the next step is to accurately
quantify and validate these biomolecules in large numbers of samples.
This instrument allows for highly selective and sensitive quantitative
measurements through specialized scan modes unavailable on other
instruments in the core (selected reaction monitoring, multiple reaction
monitoring as well as linked precursor, product, and neutral loss