Vol 1 - Spring 2011



Welcome to our first OSUCCC Shared Resources Quarterly Newsletter. Our goals are to provide you with important updates on our shared resources such as new equipment or capabilities, to highlight the excellent science resulting from each shared resource and to keep you updated on how we provide these services. After reviewing this newsletter, please take a few moments to tell us what you think. Click here to take the survey.


SharedHighlights



lairmore Michael Lairmore, DVM, PhD, Named Associate Director of Shared Resources
Michael Lairmore, DVM, PhD, a nationally respected researcher, has been promoted to lead the shared resources at The Ohio State University Comprehensive Cancer Center.
 

 


OSUCCC Receives Exceptional Rating
The National Cancer Institute (NCI) has awarded The Ohio State University Comprehensive Cancer Center the highest possible rating – “exceptional” – after completing its scientific peer review of the University’s cancer program. 

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Click on the name of the shared resource name to visit their respective web pages for more information on services, fees and contact information. 

Small Animal Imaging (SAISR)
shared resources small animal 2.JPGNEW SERVICE: Magnetic resonance microscopy (MRM) for evaluating phenotypic differences in embryos of genetically engineered mice. This technique can be used to obtain an isotropic 3D MR image of an embryo at a spatial resolution on the order of 30-40 microns. MRM can be performed on the sample prior to histologic staining of the sample and will complement the pathology review provided by the Comparative Pathology and Mouse Phenotyping Shared Resource.

Biostatistics (BSR)
NEW SERVICES: Adaptive sample size, new methods for donor cell experiments and a new mechanism for hypothesis testing. Read more

Nucleic Acid (NASR)
NEW SERVICE: New protocol for Illumina sequencing involves directional RNA (sequencing of coding + non-coding regions)-seq using the Duplex-Specific nuclease (DSN) approach. DSN normalization degrades abundant DNA molecules derived from rRNA, tRNA and housekeeping genes (which comprise > 90 percent of total RNA) while preserving DNA molecules derived from less abundant transcripts. Read more

Comparative Pathology and Mouse Phenotyping (CPMPSR)
NEW SERVICE: Aperio Scanner service provides ability to view, capture and analyze. Tired of storing hundreds of histology slides in your lab? Need a microscope to analyze and photograph them? Let us scan them using the new Aperio Scanner. 20x and 40x resolution scanning available!

Proteomics Shared Resource (PSR)
NEW SERVICE: The Proteomics and Mass Spectrometry Shared Resource has a new Protein Expression & Purification Facility. We can help you close, express and purify your proteins as well as solve problems and identify proteins to help determine your protein’s function and possible pathway.

Genetically Engineered Mouse Modeling Core (GEMMC)
GEMMC is now up and running and available to all investigators who currently work or intend to work with mouse models of human diseases. 

Analytical Cytometry (ACSR) 
NEW SERVICE: A new BD FACSAria III Flow Cytometer is coming soon! This instrument will have new laser capabilities: 488 nm, 640 nm, 561 nm and 355 nm lasers (blue, red, yellow-green and UV) and double the current sorting capability of the shared resource. Read more

Behavioral Measurement Shared Resource (BMSR)
The BMSR provides expert assistance in planning and developing research proposals involving behavioral data collection or recruitment, population data retrieval, design and coordination of qualitative and quantitative research.

Exciting new work-Heather Baltic, MPH developed a REDCap database to track call center activity on Electra Paskett's R24 (CBPR Strategies to Increase Colorectal Cancer Screening in Ohio Appalachia).  Read more

Biomedical Informatics Shared Resource (BISR)
The BISR has developed a highly efficient pipeline for processing the massive amount of nucleotide sequencing data generated by next generation sequencer Illumina GAII. Read more

Microarray Shared Resource MASR
The Microarray Shared Resource will be upgrading our Next-Generation DNA Sequencers to two state-of-the-art 5500xl SOLiD Sequencers this summer. Three years ago, we received our first SOLiD version 1 sequencer, which was the first Next-Generation Sequencer in Ohio. We soon added a second and have gone through four upgrades. The technology has advanced so rapidly in this arena that the technology of three years ago has been superseded. Read more

Microscopy Shared Resource (MSR)
shared resources Olympus.jpgNEW SERVICE: With the OLYMPUS FV1000 MULTIPHOTON MICROSCOPE, two-photon excitation provides optical sectioning with minimal photobleaching and phototoxicity above and below the plane of focus. Because two-photon excitation occurs only at the focal plane, bleaching is only confined to this area of the specimen. Consequently, this technique can be less phototoxic to live specimens.




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Medicinal Chemistry (MCSR)
Small molecules are our business! The newly developed Medicinal Chemistry Shared Resource stands ready to solve any need for small-molecule agents in your research. MCSR offers the custom synthesis of small-molecule agents with the highest quality, in a time- and cost-efficient manner.

In the past year, the MCSR has assisted OSUCCC investigators by synthesizing a number of previously published but commercially unavailable agents that target important pathways in cancer cells, and would like to make them available to the CCC community. These agents include inhibitors of ATR, Btk, Hedgehog, PI3Kdelta, RSK, MEK, TBK1, G protein-coupled receptor GPR30 and PARP. Click here for the list of agents in stock and pricing details.

Nutrient and Phytochemical Analytic Shared Resource (NPASR)
NPASR is now officially up and running! We offer expert bioanalytical method development and quantitative analysis of nutrients and phytochemicals in foodstuffs and their metabolites in biological samples as well as an enhanced understanding of the role of dietary compounds in cancer prevention and control. 

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Small Animal Imaging
Anna Bratasz, PhD, will be presenting a poster at the annual International Society for Magnetic Resonance in Medicine (ISMRM) meeting in Montreal, Canada, in May: “A New Transcriptionally Driven Oncovirus With Vstat120 Expression Has Antiangiogenic and Anti-Tumorigenic Effects” (Yoo JYet al). The focus of the poster is on the use of DCE MR noninvasive imaging for quantifying vascular leakiness and necrosis in oncolytic virus therapy for the treatment of cancer, the focus of Dr. Balveen Kaur’s laboratory. 

Niranchana Manivannan, MS, will also be presenting an oral presentation at the ISMRM meeting: “Orthogonal Super Resolution Reconstruction for 3D Isotropic Imaging in 9.4 MRI” (Manivannan N, et al.). The focus of the presentation is on the use of multiple low-resolution MR imaging views to obtain a high-resolution MR image. This high-resolution image can then be used to improve the evaluation of in vivo small animal models of cancer and other diseases.

Biostatistics
Drs. Li, Jarjoura and Muthusamy (IMM) have submitted a paper delineating the design and analysis methods that lead to strong conclusions in up-and-down regulation vs. control experiments. Drs. Jarjoura, Yu, Fernandez, Pennell and Gulati have a manuscript ready for submission for the development of a smoothing technique in variance estimation. The ordinary t-test is not powerful enough to detect true positives in an extremely large number of genes and a small sample size. The technique borrows information across genes to increase power while tightly controlling false positives.

Nucleic Acid 
Hannes Alder and Pearlly Yan are recognized as co-authors of Dr. de la Chapelle’s Science paper, “Mutations in U4atac snRNA, a Component of the Minor Spliceosome, in the Developmental Disorder MOPD.” Hannes provided consultation and data for conventional Sanger-based DNA sequencing (re-sequencing) and genotyping (SNaPshot assays) using Applied Biosystems 3730 DNA Analyzers as a validation for the NGS data. Pearlly was able to provide consultations and high throughput sequencing for the Illumina libraries generated from PCR products enriched using custom sequence-captured probes, targeting the 1.8 Mb homozygosity region of chromosome 2. Click here for the full text. For other publications featuring data generated from NASR click here.

Proteomics Shared Resource
The Complete Biosynthesis of Genetically Encoded Amino Acid Pyrrolysine From Lysine. Nature magazine. Click here for full text.  

Comparative Pathology and Mouse Phenotyping  (Also Featuring Microscopy and Biostatistics)
IL-12 Enhances the Antitumor Actions of Trastuzumab via NK Cell IFN-{gamme} Production. Journal of Immunology. Click here for full text.

SharedSpotlight
chen.JPGChing-Shih Chen, PhD, is the Lucius A. Wing Chair of Cancer Research and Therapy and professor of medicinal chemistry, of internal medicine and of urology. He has received an Ohio State University Distinguished Scholar Award, which recognizes exceptional scholarly accomplishments by senior professors. Dr. Chen’s research focuses on exploring signaling pathways that govern cancer cell survival as targets for drug discovery and design. He employs a multidisciplinary approach to identify new molecular targets by combining expertise ranging from synthetic organic chemistry to molecular and cellular biology in his research team. Read more

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OSUCCC Anniversary Celebration
Mark your calendar for a celebration of the 35th anniversary of The Ohio State University Comprehensive Cancer Center (OSUCCC) to be held from 12:30-2 p.m. Wednesday, May 25, in the grass area behind the Biomedical Research Tower. Please click here to RSVP.
admin web updates.JPG
This fall, we anticipate the rollout of our new AdminWeb ordering system. This will allow the end user to enter and track orders for OSUCCC Shared Resources online by using your name. # login. This system will put us in compliance with the University’s new eRequest mandate.  Updates and training sessions will be scheduled as the rollout date gets closer. Please contact Veronica Rice, administrative manager of shared resources with any questions or comments.

The Ohio State University Comprehensive Cancer Center – Arthur G. James Cancer Hospital and Richard J. Solove Research Institute (OSUCCC – James) 300 W. 10th Ave. Columbus, OH 43210 Phone: 1-800-293-5066 | Email: jamesline@osumc.edu