Vol 4 - Summer 2013


Welcome to Ohio State’s Comprehensive Cancer Center – James Cancer Hospital and Solove Research Institute Shared Resources Quarterly E-Newsletter. Our goal is to provide you with important updates on our shared resources, such as new equipment and capabilities, to highlight the excellent science resulting from each Shared Resource, and to keep you updated on how we provide these services. We appreciate your feedback at veronica.rice@osumc.edu.




In anticipation of a new fiscal year, we have recently completed a full cost analysis for each of our Shared Resources (SR). What does this mean? It means that we have thoroughly gone through every service provided by our SRs, and have compiled the actual cost to provide each respective service. As we begin the new fiscal year on July 1, 2013, please note that many of the prices within each SR have changed. While some did increase, some also decreased. If you have any concerns, questions, or requests concerning this, please contact Veronica Rice,veronica.rice@osumc.edu. Visit the SR landing page for fee information.

As the name Shared Resource implies, our facilities are set up so that users of each SR are essentially sharing the expenses associated with the respective service. By sharing these costs, each principle investigator (PI) does not have to duplicate the equipment, expertise or space. It also means that the cost passed along to each user must be treated on a same fee for same service basis – thereby charging each user of a facility an equitable amount. We abide by this, as this is a federal regulation. Please note that because of this, we cannot provide discounts.

eRamp Update

eRamplogoNEW cores will be coming on board in eRamp very soon. Look for the CCTS Clinical Research Center (CRC) and Mass Spec & Proteomics (MS&P) in the near future. Anyone requiring training or with questions on how to use the system, please contact Heather Richards at heather.richards@osumc.edu or visit eramp.osumc.edu. 

Over the next several months, our IT team will be working on an eRamp upgrade. Please stay tuned for further information!

Publication Tracking
Coming in August, we will be deploying a new PI Publications service. This will allow PIs to access their pubs in a web-based format and verify that all pertinent information including PMCID is captured. It will also allow them to attribute usage from a SR as contributing to their publication.




Medicinal Chemistry Shared Resource (MCSR)

Michael DarbyMedicinal Chemistry has added a new staff member to help decrease turnaround times. The OSUCCC – James and College of Pharmacy have each contributed funding in order to bring on board the expertise of Michael Darby, PhD.




Small Animal Imaging Shared Resource (SAISR)

IVIS Lumina IIIn Vivo Bioluminescent Imaging now available in BRT!
An IVIS Lumina II from Perkin Elmer (previously Caliperl Life Sciences) is now currently available in the Small Animal Imaging Core Facility located in the basement of the BRT. This system is an optical imaging system that can be used for both in vivo bioluminescent and fluorescent imaging. The system includes a highly sensitive CCD camera, light-tight imaging chamber and complete automation and analysis capabilities. Information on access to and training on this instrument can be obtained from Kim Powell, Kimerly.Powell@osumc.edu, or Anna Bratasz, Anna.Bratasz@osumc.edu.



Mass Spec&Proteomics (MS&P)
MS&P has acquired a triple quadrupole mass spectrometer. Proteomics and Metabolomics have two distinct experimental pathways, discovery and targeted. Previously, the instrumentation available in the MS&P was all discovery-based by mass spectrometer, in order to perform targeted analysis a lab must use a triple quadrupole mass spectrometer. Previously there was no existing triple quadrupole or related instrument in the CCIC MS&P lab that could be used to perform quantitative proteomics and metabolomics in a high throughput manner, and therefore could only be used for protein discovery. To qualify and validate these proteins in large numbers of samples, a triple quadrupole mass spectrometer was acquired. This instrument allows for highly selective and sensitive quantitative measurements through specialized scan modes unavailable on other instruments in the core (selected reaction monitoring, multiple reaction monitoring as well as linked precursor, product, and neutral loss scans).

Nucleic Acid Shared Resource (NASR)
Illumina Update
The new HiSeq 2500 has arrived in our NASR-Illumina core. For information, please contact Pearlly Yan, PhD, 688-8462 or email pearlly.yan@osumc.edu.

Improved Turnaround Times for Microarray Services
Recent process improvements have led to shorter turnaround times for microarray services provided by the OSUCCC – James’ NASR – Microarray Shared Resource, a multifunctional resource that leverages the research expertise of its staff with state-of-the-art genomics instrumentation to provide researchers with cost-effective, high-quality data. The facility lab specializes in gene expression with the capability for whole genome, whole transcriptome, exome and miR expression profiling. Current expertise includes library preparation and next generation sequencing, commercial microarrays and custom-built microRNA arrays. For more information about this shared resource or to discuss your upcoming research, contact Sarah Warner, lab manager, at 292-2442, Pearlly Yan, PhD, 688-8462 (sequencing) or Hansjuerg Alder, PhD, 292-6047 (gene expression analysis).

Real Time PCR Update
We have a new staff member in the NASR – RTPCR. Hopefully our turnaround times will improve.

A new QuantStudio™ 12K Flex system adds extra capacity to the NASR’s three Applied Biosystems 7900HT Fast Real-Time PCR Systems and its two Applied Biosystems StepOnePlus Real-Time PCR Systems. Extra capacity will be reflected in the OpenArray format for effortless scaling from 1 to 12,000 data points in a single run and the simple OpenArray workflow including the Accufill System. For more information, please contact Hansjuerg Alder, PhD, 292-6047.

NanoString Update
The nCounter System (NanoString Technologies) new single-cell expression assay provides ultra-sensitive, reproducible and highly multiplexed gene-expression profiling from single cells or with as little as 10pg of total RNA. The assay linearly amplifies up to 800 target transcripts from a single cell in a single tube without bias. For more information, please contact Hansjuerg Alder, PhD, 292-6047.

Space, Operations and Projects (CCCLabs)
The Space, Operations & Projects team has a new website. This site will be updated as needed, but you will already find a lot of useful information concerning:

  1. Building Access (requirements, the process involved, forms…)
  2. Communications including Newsletter archives with a topic index. These can be very helpful especially as it concerns safety and EHS reminders.
  3. Equipment: policies for shared equipment, equipment disposal and transfer forms, and the shared equipment list — broken down by type, building, and floor
  4. Polaris shuttle schedules and necessary forms
  5. Building Issues (how to report issues or concerns and improve response times)

Coming soon: contact lists, safety and training sections.

There are a couple of ways to access the site.

  1. Click this link to go directly to webpage.
  2. Visit http://cancer.osu.edu and select Researchers and Health Care Professionals, Laboratories & Facilities, and select Space, Operations and Projects (CCCLabs) tab.

Small Animal Imaging (SAISR) –

In addition to adding the new IVIS bioluminescence machine, SIASR will be demoing an Olympus Bioluminescence Microscope that complements the IVIS.  For details, please contact Dr. Kim Powell at Kimerly.powell@osumc.edu.

Microscopy (CMIF)–

Microscopy (CMIF) will be hosting a demo of Super Resolution Confocal Microscope coming later this summer!  Please contact john.robinson2@osumc.edu; sara.cole2@osumc.edu, or Montione.1@osu.edu for further information.

Analytical Cytometry/Flow (ACSR)–

Analytical Cytometry/Flow (ACSR) will be hosting a two day training session, hosted by external experts.  For more information, please contact bryan.mcelwain@osumc.edu.

Change for Mass Spectrometry and Proteomics (MS&P)-

The Mass Spectrometry and Proteomics Facility (MS&P) has acquired a Waters Xevo-TQ-S triple quadruple mass spectrometer complete with a nano-Acquity 2d UPLC system.  Proteomics and Metabolomics have two distinct experimental pathways for quantization of biomolecules, discovery and targeted. Historically, the instrumentation available in the MS&P was all discovery based.  Targeted metabolomic and proteomic analysis utilizes a triple quadrupole mass spectrometer.  Previously there was no existing triple quadrupole in the MS&P lab that could be used to perform targeted quantitative proteomics and metabolomics in a high throughput manner.  Now the MSP can perform both discovery based and targeted based proteomics and metabolomics.  After a biomolecule of interest is discovered, the next step is to accurately  quantify and validate these biomolecules in large numbers of samples.  This instrument allows for highly selective and sensitive quantitative measurements through specialized scan modes unavailable on other instruments in the core (selected reaction monitoring, multiple reaction monitoring as well as linked precursor, product, and neutral loss scans).

The Ohio State University Comprehensive Cancer Center – Arthur G. James Cancer Hospital and Richard J. Solove Research Institute (OSUCCC – James) 460 W. 10th Avenue, Columbus, OH 43210 Phone: 1-800-293-5066 | Email: jamesline@osumc.edu