The Pharmacoanalytical Shared Resource supports pre-clinical and clinical drug development at OSUCCC–James by providing high quality, cost-effective and reliable bioanalytical method development, quantitative sample analysis, and pharmacokinetic/pharmacodynamic/pharmacogenetic experimental design and data analysis.
Primary PhASR equipment comprises three liquid chromatography/mass spectrometry (LC-MS) systems, which include a quadrupole time-of-flight (Q-tof) and two triple-quadrupole mass spectrometers. One triple quadrupole instrument is supported by an ultra-high pressure liquid chromatography (UHPLC) system designed for high-throughput sample analysis, and the other two are supported by high pressure liquid chromatography (HPLC) systems.
Liquid Chromatography/Mass Spectrometry (LC-MS) System Details:
ThermoFinnigan TSQ Quantum Discovery Max: a triple-quadrupole mass spectrometer with ESI and APCI ionization probes and a ThermoFisher Acella ultra-high performance LC system (degasser, quaternary pumps, and photo-diode array detector), which includes an autosampler that is expandable for multiplexing, online extraction and high-throughput analysis
ThermoFinnigan TSQ Quantum Ultra: a triple-quadrupole mass spectrometer with extended mass range connected to a Shimadzu Prominence LC system (with ternary pumps, degasser, temperature-controlled autosampler and column compartment)
Micromass Q-Tof Micro Hybrid Quadrupole/Orthogonal TOF-MS: with Lockspray Electrospray and a Waters 2795 Alliance HT HPLC (ternary pump, temperature-controlled autosampler and column compartment)
Additional LC/MS systems (ion traps and triple-quadrupole mass spectrometers) are housed in the College of Pharmacy investigator laboratories and are available for PhASR use.
PhASR also maintains full access to other resources necessary to develop and run biological correlative assays, including UV-Vis and fluorescence spectrometers, plate-readers, thermal cyclers, centrifuges, cell culture and animal facilities, and freezer and refrigerator storage.
WinNonlin and NONMEM Pharmacokinetic Analysis Software Packages are also available for data analysis.