The Small Animal Imaging Center Shared Resource (SAISR) facility houses high-resolution small animal imaging instrumentation and offers assistance from experts in: operating each imaging modality, small animal handling procedures and analytical software support for quantitative image analysis.
The following micro-imaging equipment includes magnetic resonance imaging (MRI), positron emission tomography (PET), single-photon emission computed tomography (SPECT), X-ray computed tomography (X-ray CT), high-frequency ultrasound (US) and electron paramagnetic resonance (EPR) imaging.
All imaging instrumentation comes fully equipped with biological (ECG, respiration, temperature) monitoring equipment and isoflurane anesthesia systems. The instrumentation can be used for in vivo live animal imaging or ex vivo specimen investigations.
The AVANCE™500 WB NMR Spectrometer & Imaging System is an 11.7T vertical bore magnet that operates at 500 MHz and runs ParaVision 4.0 software. The system maintains ultra-shielding technology, which provides maximum field homogeneity, field stability and stray field reduction. The 8.9 cm diameter bore is best suited for whole-body mouse imaging.
Equipped with 3 RF channels (proton NMR, deuterium and broadband) and preamplifiers, the AVANCE™500 has two sets of gradient coils, MicroMouse in vivo imaging probes, BioTrig monitoring units and an in vivo cardiac gating system.
MRI applications include:
- Skeletal and soft tissue abnormalities in embryonic and adult specimens
- DCEMRI for tumor vascularization and permeability
- In vivo spectroscopy to measure metabolites
- Cardiac imaging for assessing cardiac function, ischemia and infarcts
The BioSpec 94/30 Imaging System is a 9.4T horizontal bore magnet that operates at 400 MHz and runs ParaVision™ 4.0 software. This microMRI system is designed for high-resolution MR spectroscopy and imaging of in vivo or ex vivo specimens.
The 20 cm diameter bore and extra long table offers imaging capabilities of 1H, 13C, 19F, 31P on mice, rats, small rabbits and ex vivo specimens. The BioSpec also includes a BioTrig monitoring unit and in vivo cardiac gating.
MRI applications include:
• Skeletal and soft tissue abnormalities in embryonic and adult specimens
• DCEMRI for tumor vascularization and permeability
• In vivo spectroscopy to measure metabolites
• Cardiac imaging for assessing cardiac function, ischemia and infarcts
The Vevo 2100 High-Frequency Ultrasound System is capable of 2D and 3D in vivo imaging in small animals, has a 30-micron spatial resolution and frame rates of up to 1000 fps. The system includes an 18, 24, 38 and 55 MHz transducer, needle-guided injection apparatus and is capable of color, pulsed wave and power Doppler, 3D imaging and advanced image analysis.
The Vevo can be used for:
• Image-guided injections
• Evaluating developmental biology – mouse and rat embryos
• Calculating tumor size and blood flow\Quantitative evaluation of cardiac physiological function and development
The Inveon™PET/CT Imaging System is a 10 cm diameter bore system. The PET system has an axial FOV of 12.7 cm and FWHM spatial resolution < 1.4 mm.
The microCT system is capable of spatial resolutions of < 0.05 mm and is equipped with a real-time reconstruction engine and post-processing workstations; Visage™volume visualization software and TRI/3D-Bon™ 3D bone morphometry software; and FEM Solver™ finite element modeling software.
PET applications include:
• 18FDG-glucose metabolism
• Mouse xenograft models, tumors and metastasis
• Alzheimer’s disease and stroke
• Myocardial studies
• 18FLT monitor cellular proliferation
• 124I-labeled antibodies localize tumors and monitor tumor burden in xenograft models
CT applications include:
• Skeletal abnormalities in embryonic and adult specimens
• Monitoring trabecular architecture and bone mineral density and content
• Liposomal iodinated contrast agents used in liver, cardiac and vasculature
The Inveon™SPECT/CT Imaging System is a dual-modality system combining PET with X-ray CT. This imaging system, which can be used for dual or single modality imaging, has a 10 cm diameter bore system and can accommodate animals up to the size of a small rabbit.
The Inveon SPECT/CT can image a variety of radionuclides, such as 18F, 15O, 13N and 11C. It has two detector heads, a detectable energy range of 30-300 keV and a maximum spatial resolution of ≤ 1 mm.
SPECT applications include:
• Monitoring tumor progression and spread in vivo
• Tumor staging and molecular profiling including cellular proliferation, apoptosis, angiogenesis, hypoxia and receptor status
• Assessing therapeutic response
• Facilitating new drug development by determining bio-distribution and retention in vivo
• Evaluating and optimizing gene delivery efficacy by including imaging reporter genes
A CRITM Maestro in vivo Fluorescence Imaging System is available in 0080 BRT through Michell.Carlton@osumc.edu