Co-Director: Kevin Weller
Co-Director: Bei Liu, MD, MPH
Lab Manager: Bryan McElwain, MS
Mass Cytometer: Greg Behbehani, MD, PhD
The FCSR provides comprehensive services for all needs of the OSUCCC – James members involving cell immunophenotyping and cell sorting. OSUCCC – James members enjoy priority access to technical and scientific training for instrumentation usage, experimental design, troubleshooting and optimization on state-of-the-art equipment maintained at the highest QC/QA standards.
The FCSR is supported by the OSUCCC – James as a crucial shared resource central to the research goals of all scientific programs in the OSUCCC – James. Investigators work with FCSR staff in a collaborative fashion to achieve their research objectives and drive development of innovation for both instrumentation development and use, as well as for novel experimental methodologies continually under development.
The FCSR supports OSUCCC – James member training at all levels — from undergraduates to principal investigators — to continually build the foundation and expansion of knowledge and skills related to flow cytometry. The FCSR staff members are routinely engaged as collaborators in OSUCCC – James member research projects, and they are recognized for these efforts by co-authorship on high-impact factor publications and by assistance in preparing data and material for new funding opportunities to support the acquisition of new funding for cancer research.
Key program objectives
The FCSR continues to strive to be a state-of-the-art cell analysis laboratory and has the following Specific Aims:
- Aim 1: Provide state-of-the-art equipment and support for high-quality cancer research for OSUCCC – James members on a fee-for-service basis. Specifically, FCSR provides instrumentation, training, and technical operation/support for all flow cytometry-based projects.
- Aim 2: Continuously work with OSUCCC – James members to provide substantial technical expertise and training for state-of-the-art cytometry instruments and panel designs to address fundamental scientific questions in cancer research.
Services
- Support and access for all OSUCCC needs related to analytical cytometry (Cytek Aurora and Northern Lights, BD Fortessa and SB Helios Mass Cytometer and cell sorting (Cytek CS and BD FACSAria) on a 24/7/365 level
- Assess up to 64 biomarkers simultaneously using spectral cytometry analytical instrumentation, 70+ biomarkers using mass cytometry, and sort up to 64 biomarkers with equipment configured to meet the recommended guidelines of the International Society of Analytical Cytometry to meet BSL2+ conditions if needed
- Specialized services for imaging cytometry (Cytek Imagestream) that images single cells and small particles in flow to gain signal localization information while simultaneously performing traditional flow cytometry measurements
- Scientific seminars, cancer-specific meetings, instrumentation overviews, application workshops and full day training sessions for researchers to learn to use and apply flow cytometry
- Lab focused-training and troubleshooting upon request for personalized support as needed
Contact FCSR
Flow Cytometry Shared Resource
1055/1063 Biomedical Research Tower
460 W. 12th Ave.
Columbus, OH 43210
To contact the FSCR leadership, please email Bei.Liu@osumc.edu and Kevin.Weller@osumc.edu.
To contact the facility, call 614-292-3569 (FLOW) or email Bryan.McElwain@osumc.edu.
This Shared Resource is supported by the Cancer Center Support Grant (P30CA016058).