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Genomics

Equipment

Learn about the equipment provided by the Genomics Shared Resource (GSR) to OSUCCC – James cancer researchers.

    Place an Order in eRAMP

    The GSR provides a centralized, comprehensive resource of high-throughput genomics technologies to OSUCCC – James researchers and non-CCC members.

    SANGER Sequencing, SHAPE, Microsatellite Genotyping and Cell Line Verification

    • Applied Biosystems 3730xl DNA analyzers

    Quality Control

    • Agilent 2100 Bioanalyzer and 4200 TapeStation – DNA and RNA integrity and size
    • Invitrogen Qubit Fluorimeter – DNA/RNA Mass

    Next-Generation Sequencing Library Generation

    Single Cell Sequencing

    • 10X Genomics Chromium Controller for 3’ and 5’ mRNA, Genomic VDJ, ATAC-seq
    • 10X Genomics Chromium X for high through-put single-cell RNA-seq
    • Formulatix Mantis and TTP Mosquito microfluidics systems for “Plate-Seq” single-cell and limited cell libraries

    Other Equipment for Library Preparation

    • Covaris E220 sonicator
    • gentleMACS Octo Dissociator (available for user sign-up)

    Equipment for Hybridization-Based Expression, Genotyping and Copy Number Assays

    • Affymetrix for RNA and miRNA expression profiling microarrays
    • nCounter system (NanoString Technologies) for gene expression miRNA expression, CNV and SNP assays Karyotype Panel and other custom-based assays

    Equipment for PCR-Based Expression, Copy Number and Genotyping Assays

    • Real-Time PCR (qPCR) (QuantStudio 5K and QuantStudio 12K Flex Systems) for gene expression (mRNA) analysis (TaqMan and SYBR Green Assays), microRNA and noncoding RNA analysis, genetic variation analysis (SNP, CNV), TaqMan mutation detection assays (castPCR), TaqMan protein assays, digital PCR
    • BiomarkHD-Juno system for Gene expression (mRNA) analysis (TaqMan and EvaGreen Assays), microRNA and noncoding RNA analysis (TaqMan), genetic variation analysis (TaqMan SNP and CNV assays, Fluidigm SNPType assay), TaqMan mutation detection assays (castPCR), TaqMan protein assays, digital PCR
    • BioRad QX200 Digital Droplet PCR system for absolute quantification of target DNA or RNA molecules using EvaGreen or TaqMan probes

    Equipment for Spatial Transcriptomics

    • NanoString Technologies GeoMx Digital Spatial Profiler for spatial transcriptomics (targeted up to 18,000 genes) and spatial proteomics (targeted)
    • 10X Genomics CytAssist supporting 10X Genomics Visium spatial profiling