Rapid Column-Free Enrichment of Mononuclear Cells from Solid Tissues.
Scoville SD, Keller KA, Cheng S, Zhang M, Zhang X, Caligiuri MA, Freud AG
Sci Rep 5 12490 07/30/2015
We have developed a rapid negative selection method to enrich rare mononuclear cells from human tissues. Unwanted and antibody-tethered cells are selectively depleted during a Ficoll separation step, and there is no need for magnetic-based reagents and equipment. The new method is fast, customizable, inexpensive, remarkably efficient, and easy to perform, and per sample the overall cost is less than one-tenth the cost associated with a magnetic column-based method.Full Text