Expertise

The ACSR provides substantial technical expertise and training for state-of-the-art cytometry instruments in order to address fundamental cancer research questions. Through a variety of educational outreach activities, OSUCCC members are introduced to new instrumentation, technology and methodologies being developed at Ohio State and other key allied organizations.

Director: Jeffery Chalmers, PhD        
Manager: Bryan McElwain, BS, MS, MBA
Lab Services Coordinator: Alex Cornwell, BS   

Jeffrey Chalmers, PhD, Director of the Cytometry Shared Resource, is also a professor in Ohio State’s Department of Chemical and Biomolecular Engineering and an adjunct staff member for the Cleveland Clinic’s Department of Biomedical Engineering, Research Institute. 

He earned his BA in Chemical Engineering from Westmont College; his BS in Chemical Engineering from the University of California, Berkeley; and his PhD in the same field of study from Cornell University. 

In addition to being the director of Analytical Cytometry Shared Resource, Dr. Chalmers serves on the Biotechnology and Bioengineering Editorial Board, and associate editor of PLoSONE, and served a term as an NIH ISD study section member. An elected Fellow of the American Association for the Advancement of Science and an elected Fellow of the American Institute of Biomedical and Biological Engineers, Dr. Chalmers has received numerous professional awards and distinctions, including the 2014 Cell Culture Engineering Award. He has also published more than 120 peer-reviewed papers, 11 book chapters and one book.

Bryan McElwain, BS, MS, MBA, is the ACSR Manager. He received his Bachelor of Science degree from St. Bonaventure University and his MS from the State University of New York at Buffalo. He studied cytometry during his master’s thesis on immunology at the Roswell Park Cancer Institute. 

Bryan has been managing the BRT core facility since 2004, gaining professional experience in designing, consulting and training facility on cytometry applications, experimental design and instrument training. He has expertise on the BD FACS Aria, BD FACS Calibur, BD FACS LSRII, and the BD FACS Vantage.

Alex Cornwell, BS, is the Lab Services Coordinator. He received his Bachelor's degree in Biology and Physics from Capital University in 2013. Alex has worked for the Analytical Shared Resource since 2014 and has extensive experience in experimental design, cytometry applications and training. He has expertise on the BD FACS Arias, BD FACS Calibur, BD FACS LSR II and Malvern Nanosight.

Instrumentation and Equipment

The ACSR currently hosts a wide array of cytometers and cell sorting instrumentation. Below are some comprehensive tables to help select the appropriate instrument for your experiment.

Cell sorters:

The shared resource has two cell sorters available: the Aria IIu and Aria III. The Aria IIu has two lasers, a 488 blue and 633 red and detectors for up to seven colors. The Aria III adds additional 355 UV and 561 yellow, green lasers along with additional detectors, taking it up to eleven colors. These lasers allow the Aria III to perform applications, including side populations, using Hoescht dyes, fluorescent proteins like mCherry, and cell ploidy. Both are capable of sorting up to four populations at a time, and plate sorting including, but not limited to, 96 wells.

Analyzers:

The shared resource has two analyzers, a two-laser four color FACSCalibur and a three- laser 12-color LSR II. Both instruments have 488 and 633 lasers, while the LSR II has an additional 405 violet laser. The violet laser and the accompanying additional detectors give you an additional wide variety of color options for your analysis applications.

Malvern Nanosight NS300:

The shared resource has a NanoSight NS300 with 532nm excitation source available for either light scattering (no labeling required) or fluorescent analysis of the sample.  The results produce a number count and size distribution of the nanoparticles in suspension.  Single fluorophore labeling (for biomarker identification, drug/cargo loading, or sizing in complex media) can be used for fluorophores with excitation near 532nm.

Flurochrome guide to selecting instrumentation:

flurochrome

Assay guide to selecting instrumentation:

​Aria III

​Aria IIu

​Calibur

​LSR II

​1 Color Assay

​X

​X

​X

​X

2-4 Color Assay​

​X

​X

​X

​X

5-8 Color Assay​

​X

​X

​X

​UV Applications

​X

mCherry/RFP​

​X

Plate Sorting​

​X

​X

Multi-population sorting​

​X

​X

 

Contact ACSR

Bryan McElwain
Laboratory Manager
Bryan.McElwain@osumc.edu


Katrina Miller
Laboratory Coordinator
Katrina.Miller@osumc.edu


BRT Facility
1055/1063 Biomedical Research Tower
460 West 12th Ave.
Columbus, OH 43210


Phone: 614-292-FLOW (3569)

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