Expertise

The ACSR provides substantial technical expertise and training for state-of-the-art cytometry instruments in order to address fundamental cancer research questions. Through a variety of educational outreach activities, OSUCCC members are introduced to new instrumentation, technology and methodologies being developed at Ohio State and other key allied organizations.

Director: Jeffery Chalmers, PhD        
Manager: Bryan McElwain, BS, MS, MBA
Lab Services Coordinator: Alex Cornwell, BS   

Jeffrey Chalmers, PhD, Director of the Cytometry Shared Resource, is also a professor in Ohio State’s Department of Chemical and Biomolecular Engineering and an adjunct staff member for the Cleveland Clinic’s Department of Biomedical Engineering, Research Institute. 

He earned his BA in Chemical Engineering from Westmont College; his BS in Chemical Engineering from the University of California, Berkeley; and his PhD in the same field of study from Cornell University. 

In addition to being the director of Analytical Cytometry Shared Resource, Dr. Chalmers serves on the Biotechnology and Bioengineering Editorial Board, and associate editor of PLoSONE, and served a term as an NIH ISD study section member. An elected Fellow of the American Association for the Advancement of Science and an elected Fellow of the American Institute of Biomedical and Biological Engineers, Dr. Chalmers has received numerous professional awards and distinctions, including the 2014 Cell Culture Engineering Award. He has also published more than 120 peer-reviewed papers, 11 book chapters and one book.

Bryan McElwain, BS, MS, MBA, is the ACSR Manager. He received his Bachelor of Science degree from St. Bonaventure University and his MS from the State University of New York at Buffalo. He studied cytometry during his master’s thesis on immunology at the Roswell Park Cancer Institute. 

Bryan has been managing the BRT core facility since 2004, gaining professional experience in designing, consulting and training facility on cytometry applications, experimental design and instrument training. He has expertise on the BD FACS Aria, BD FACS Calibur, BD FACS LSRII, and the BD FACS Vantage.

Alex Cornwell, BS, is the Lab Services Coordinator. He received his Bachelor's degree in Biology and Physics from Capital University in 2013. Alex has worked for the Analytical Shared Resource since 2014 and has extensive experience in experimental design, cytometry applications and training. He has expertise on the BD FACS Arias, BD FACS Calibur, BD FACS LSR II and Malvern Nanosight.

Instrumentation and Equipment

The ACSR currently hosts a wide array of cytometers and cell sorting instrumentation. Below are some comprehensive tables to help select the appropriate instrument for your experiment.

Sorting Instruments:

Becton Dickinson FACSAria IIu: The Aria IIu has two lasers, a 488 blue and 633 red and detectors for up to seven colors.

Becton Dickinson FACSAria III: The Aria III has four lasers, a 488 nm, 633 nm, 355 nm, and 561 nm for detection of up to eleven colors. These lasers allow the Aria III to perform applications, including side populations, using Hoescht dyes, fluorescent proteins like mCherry, and cell ploidy. Both are capable of sorting up to four populations at a time, and plate sorting.

Analysis Instruments:

Becton Dickinson LSRFortessa Flow Cytometer: Equipped with five laser lines (355nm, 405nm, 488nm, 561nm, and 640nm) and is capable of detecting 18-color parameters. The instrument is also equipped with a High Throughput Sampler (HTS) that is compatible with 96-welll plates as well as 384-well plates, for fully automated sample acquisition.

Becton Dickinson LSR II Flow Cytometer: Equipped with three lasers—the 488nm, 633nm, and 405nm laser lines and is capable of detecting 12-color parameters. This instrument delivers additional sensitivity and resolution beyond the FACScaliber for multicolor applications as it may detect up to 12 color parameters simultaneously.

Becton Dickinson FACSCalibur Flow Cytometer: Equipped with two lasers (488nm & 640nm) for up to four-color analysis. The BD FACSCalibur offers a modular approach to flow cytometry and allows users to perform simple cell analysis experiments such as cell cycle analysis.

Amnis ImageStreamX Mark II Imaging Cytometer: Equipped with four lasers and imaging capabilities for up to 9-color, two Brightfield, and one Scatter parameters simultaneously. The ImageStream combines the speed and sensitivity of flow cytometry with the detailed imagery and insight of microscopy. The ImageStream allows for extensive range of applications such as co-localization, shape change, internalization, advanced cell cycle analysis, etc.

Malvern NanoSight NS300 allows for analysis of the size distribution and concentration of all types of nanoparticles from 10 nm to 2000 nm in diameter, depending on the instrument configuration and sample type. The Nanosight contains a 532 nm laser and one optional 565/20 detector to quantify fluorescent particles (Fluorescent labeling is not required). Each particle’s Brownian motion is individually but simultaneously analyzed by direct observations of diffusion.

 Instrument      Aria III    
     Aria      FACS Calibur    LSR III     LSR Fortessa 
ImageStream X Mk II 
 Lasers 355       355  
    405 405 405
488 488 488 488 488 488
561       561 561
633 633 633 633 633 633
 Common
 Fluorochromes
          785 (SSC)
 
 Hoescht X       X  
 Indo X       X  
 DAPI X     X X X
 Pacific Blue       X X X
 AF 405       X X X
 Amcyan       X X X
 AF 430       X X X
 QD 565         X X
 QD 585       X X X
 BV 605       X X X
 QD 655       X X X
 QD 705         X X
 QD 800           X
 AF 488 X X X X X X
 FITC/GFP/YFP X X X X X X
 PE X X X X X X
 PE - Texas Red X X X X X X
 PE-CY5 X X X X X X
 PE-CY7 X X X X X X
 PerCP-CY5.5 X X X X X X
 Propridium Iodide X X X X X X
 7AAD X X X X X X
 mcherry X       X X
 mTomato X       X X
 AF 647 X X X X X X
 APC X X X X X X
 AF700 X X X X X X
 APC-CY7 X X X X X X

Assay guide to selecting instrumentation:

                                 Aria III    Aria   Calibur  LSR II  Fortessa ImageStream
X Mk II
NanoSight
NS300
 1 Color Assay X X X X X X  
 2-4 Color Assay X X X X X X
 5-8 Color Assay X X   X X X  
 9-12 Color Assay         X    
Nanoparticle/
Extracellular Vesicle
Application
          X X
Cell Imaging
Applications
          X  
 UV Applications X       X  
 mCherry/RFP X       X X  
 Plate Sorting X X          
Multi-Population
Sorting
X X          

Contact ACSR

Bryan McElwain
Laboratory Manager
Bryan.McElwain@osumc.edu


Alex Cornwell
Laboratory Coordinator
Alex.Cornwell@osumc.edu


BRT Facility
1055/1063 Biomedical Research Tower
460 West 12th Ave.
Columbus, OH 43210


Phone: 614-292-FLOW (3569)

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