Director: Vincenzo Coppola, MD
Modeling human disease in mouse provides a powerful tool for elucidating mechanisms and interrogating experimental therapeutics. The Genetically Engineered Mouse Modeling Core (GEMMC) is a state-of-the-art shared resource offering a complete array of services available to investigators who are currently working or intend to work with mouse models of human disease.
The GEMMC team has extensive experience in generating and maintaining genetically engineered mouse lines and is available to assist investigators with minimal experience who want to start using mouse models to complement and strengthen their in vitro studies or grant proposals.
The GEMMC team can assist in producing classical transgenic lines by pronuclear injection of DNA and targeted lines (knock-out, knock-in, conditional) by CRISPR/Cas9 and by embryonic stem (ES) cell micromanipulation.
The generation of new mouse mutant lines by CRISPR/Cas9 is an "all-inclusive" service that includes designing of the targeting strategy, screening of potential founder (F0) animals and breeding to F1 germ line-transmitted mutant allele.
On trial and collaborative bases, the GEMMC can produce crisprized human and mouse cell lines.
The GEMMC also offers all other ancillary services, including sperm or embryo cryopreservation, rederivation and in vitro fertilization (IVF).
Investigators new to The Ohio State University are strongly encouraged to reach out to us for their needs and consultation. It is desirable to have an introductory meeting to illustrate in a more detailed manner our services.