Director: Vincenzo Coppola, MD
Modeling human disease in mouse provides a powerful tool for elucidating mechanisms and interrogating experimental therapeutics. The Genetically Engineered Mouse Modeling Core (GEMMC) is a state-of-the-art shared resource offering a complete array of services available to investigators who are currently working or intend to work with mouse models of human disease.
The GEMMC team has extensive experience both in generating and maintaining genetically engineered mouse lines and is available to assist investigators with minimal experience who want to start using mouse models to complement and strengthen their in vitro studies or grant proposals.
The GEMMC team can assist in producing classical transgenic lines by pronuclear injection of DNA and targeted lines (knock-out, knock-in, conditional) by embryonic stem cell technology and by CRISPR/Cas9 technology. CRISPR services include designing of the targeting strategy and screening of F1 germ line-transmitted mutant mice.
On trial and collaborative bases, the GEMMC can produce crisprized human and mouse cell lines.
The GEMMC also offers all other ancillary services, including sperm or embryo cryopreservation, rederivation and in vitro fertilization (IVF).
- Director: Vincenzo Coppola, MD
- Assistant Director: Xin-An Pu, PhD
Vincenzo Coppola, MD, is an assistant professor in the Department of Molecular Virology, Immunology and Medical Genetics in Ohio State’s College of Medicine. He joined the Ohio State faculty in 2010 after nine years of heading the Gene Targeting Facility in the Mouse Cancer Genetics Program at the National Cancer Institute (NCI).
Dr. Coppola earned his MD at Federico II University in Naples, Italy, where he also served his medical residency. He received specialized oncology training at the University of Padua and completed a postdoctoral fellowship at the NCI.
Dr. Coppola’s personal research focuses on the role of the “adaptome” in cancer. His lab is currently studying the role of a new scaffold protein, RanBPM, which coordinates different pathways relevant to cancer.
Xin-An Pu, PhD, is an adjunct assistant professor in the Department of Neuroscience in Ohio State’s College of Medicine and a research specialist at the OSUCCC – James. She earned her PhD at the University of Missouri and completed postdoctoral training at Cornell University.