Director: Richard K. Wilson, PhD
Co-Director: Amanda Ewart Toland, PhD, FACMG
Technical Director (Ohio State): Pearlly Yan, PhD
Technical Director (Nationwide Children’s Hospital): Vincent Magrini, PhD
The Genomics Shared Resource (GSR) provides a centralized, comprehensive resource of high-throughput genomics technologies to OSUCCC – James researchers and non-CCC members.
The GSR offers instrumentation and expertise for DNA and RNA analysis using sequencing, genotyping, real-time PCR, Affymetrix GeneChips, nCounter Analysis, next-generation sequencing library generation, next-generation sequencing (through Nationwide Children’s Hospital Biomedical Genomics Core), nucleic acid extraction and QC for RNA/DNA/proteins.
OSUCCC member and non-member investigators have unlimited access to training, consultation, troubleshooting and assistance in experimental design.
- Provide reliable, high-quality, affordable, low- and high-throughput genomic and epigenomic support
- Provide, optimize, develop and apply early access technologies relevant to cancer research
- Provide an infrastructure and staff expertise to work with users on
- Provide investigators with training in data analysis, data interpretation and experimental strategies, and provide assistance with investigator publication
- Utilize molecular hybridization methods to detect and quantify RNA transcript expression levels and types (splicing isoforms and gene fusions) and genomic structural and nucleotide variants (DNA copy number and large insertion/deletions) via digital (Nanostring) and microarray (Affymetrix) platforms
- Provide RNA and DNA quality control metrics
- Provide full service next-generation library generation for a variety of library types and input
- Perform polymerase chain reaction (PCR)-based amplification to detect, quantify and confirm copy number variants, single nucleotide variants and small insertion/deletion polymorphisms including quantitative PCR and high throughput digital and custom PCR assay methods
- Provide Sanger sequence and PCR-based single nucleotide variant and microsatellite genotyping using a variety of platforms (microsatellite genotyping, TaqMan SNP and CNV assays, Fluidigm SNPType assay and Snapshot) and needs (e.g. cell line verification, hotspot mutation analysis, case control studies)