The MSR offers the following instruments and equipment for use by members of the OSUCCC – James. Twenty-four hour access to these instruments is offered to users who demonstrate the appropriate level of proficiency.
The FEI Tecnai Spirit BioTWIN Transmission Electron Microscope (TEM) is specifically designed for examination of biological specimens. The 120 kV TEM shows the fine structure of cellular organelles, details of cell-cell interactions and the distribution of extracellular matrix. It is also used for high- resolution examination of negatively stained nanostructures to determine their size and shape.
Scanning Electron Microscopy (SEM) is a microscope that provides high-resolution, digital imaging that is ideal for examining sample surfaces. The MSR anticipates installation of a replacement system in late 2019 following flood damage to the old system.
Olympus Confocal Microscopes are used for fixed material prepared for immunocytochemistry to be examined at the highest possible resolution. All confocal systems are equipped with four lasers, four fluorescence detectors, a DIC detector and a range of objective lenses. Users receive a combination of classroom and hands-on training to prepare them for independent use of the confocal by their third session. The newly installed Olympus FV3000 is a fully automated system with five fluorescence detectors and five laser lines including a 750nm laser. This provides users with a macro to micro evaluation arrangement when used in conjunction with the IVIS imaging system available through the Small Animal Imaging Core Facility.
The Olympus FV1000 Multiphoton Laser Scanning Confocal System is equipped with a Deep See MaiTai TiSapphire laser, a 25x, 1.05 N.A water-immersion objective lens with a 2mm working distance, four non-descanned detectors and a forward, second harmonic generation (SHG) detector for collagen detection without the addition of a fluorescent marker. Infrared excitation using the two-photon system provides optical sectioning deeper than conventional confocal microscopy (up to 1mm). Because two-photon excitation occurs only at the focal plane, photobleaching is only confined to this area of the specimen. Consequently, this technique can be less phototoxic, making it ideal for live animal imaging. Additional support equipment for live animal imaging is also available, including a vital signs monitor and animal temperature control. This system also functions as a standard confocal instrument with four laser lines, three fluorescence detectors (two spectral) and a DIC detector.
The Nikon A1R live cell imaging system has four lasers with four fluorescence detectors and contrast detection (DIC). The Nikon confocal system is ideally suited for imaging rapid physiological events and has spectral imaging capabilities. This system is both a confocal and a widefield system using a highly sensitive EMCCD Hamamatsu camera. An atmospheric control chamber is available for maintaining temperature and CO2 levels. The automated stage provides X and Y control for imaging multiple positions within the culture chamber. Rapid Z-stack acquisition is accomplished with the piezo stage insert to provide the investigator with three-dimensional images over time (4D imaging).
Light Microscopes available at the MSR provide high quality, light level digital images to OSUCCC researchers. The widefield microscope is equipped with a QICLICK-F-M-12 CCD camera (QImaging) to provide bright field, dark field, phase contrast, and fluorescence capabilities. A CRI Nuance multispectral imaging camera is available for use at the MSR in conjunction with the Zeiss Axioskop microscope to assist the researcher in separating signals in samples prepared using either chromogens or fluorophores. Both cameras can be used with the Olympus SZH Zoom Stereo Microscope system for low magnification imaging of whole mount or large samples not suitable for the widefield system. The stereomicroscope has an auxiliary fluorescence light source, the Nightsea viewing system, capable of illuminating samples containing green fluorescence markers (i.e. GFP).
The ThermoFisher Cryotome E Cryostat enables the researcher to obtain 5-40 micron sections of chemically fixed tissue for examination using light microscopy.