Services


Services

The Microscopy Shared Resource (MSR) offers training tailored to the needs of the individual investigator on all microscope systems in the facility. Unique to the facility, consultation and support on subjects ranging from experimental design, troubleshooting and data analysis are also offered. Processing and analysis software for image presentation and publication is also available to Ohio State cancer researchers.

The MSR is the only facility on campus that provides processing of biological samples for evaluation using electron microscopy. These services include sample processing of TEM and SEM samples requiring different treatments in order to obtain optimal results. For SEM sample processing, the researcher can choose between chemical dehydration or critical point drying depending on the application and desired result. Traditional epoxy resin processing and sectioning are available for TEM sample observation. However, examination of soft materials such as biopolymers used as substrates for cell growth is very difficult to section using this approach. For optimal imaging of soft samples, a cryo-ultramicrotome is available to section these samples after freezing. Cryo-ultramicrotomy is also offered for observation of samples using light microscopy to assist in eliminating out of focus light, an issue that is often a result of autofluorescence. The MSR also offers negative staining as a means of observing samples when dehydration is not suitable for maintaining structure.

Instrumentation Equipment

The MSR offers the following instruments and equipment for use by members of the OSUCCC – James. 24-hour access to these instruments is offered to users who demonstrate the appropriate level of proficiency.

The FEI Tecnai Spirit BioTWIN Transmission Electron Microscope (TEM) is specifically designed for examination of biological specimens. The 120 kV TEM shows the fine structure of cellular organelles, details of cell-cell interactions and the distribution of extracellular matrix. It is also used for high- resolution examination of negatively stained nanostructures to determine their size and shape.

The FEI Nova NanoSEM is a scanning electron microscope (SEM) equipped with a field emission gun and a through-the-lens that provides high-resolution digital imaging ideal for examining sample surfaces detectors. The ultrahigh resolution of up to one half million times magnification is used to examine structures including, but not limited to, cell surfaces, extracellular matrices, biofilms and nanostructures.

Olympus Confocal Microscopes are used for fixed material prepared for immunocytochemistry to be examined at the highest possible resolution. All confocal systems are equipped with four lasers, four fluorescence detectors, a DIC detector and a range of objective lenses. Users receive a combination of classroom and hands-on training to prepare them for full use of the confocal by their third session. The newly installed Olympus FV3000 is a fully automated system with five fluorescence detectors and five laser lines including a 750nm laser (arriving in early 2018). This provides users with a macro to micro evaluation arrangement when used in conjunction with the IVIS imaging system available through the Small Animal Imaging Core Facility. The facility also provides training and access to Olympus FV1000 confocal systems.

The Olympus FV1000 Multiphoton Laser Scanning Confocal System is equipped with a Deep See MaiTai TiSapphire laser, a 25x, 1.05 N.A water-immersion objective lens with a 2mm working distance, four non-descanned detectors and a forward, second harmonic generation (SHG) detector for collagen detection without the addition of a fluorescent marker. Infrared excitation using the two-photon system provides optical sectioning deeper than conventional confocal microscopy (up to 1mm). Because two-photon excitation occurs only at the focal plane, photobleaching is only confined to this area of the specimen. Consequently, this technique can be less phototoxic, making it ideal for live animal imaging. Additional support equipment for live animal imaging is also available, including a vital signs monitor and animal temperature control. This system also functions as a standard confocal instrument with four laser lines, three fluorescence detectors (two spectral) and a DIC detector.

The Nikon A1R live cell imaging system has four lasers with four fluorescence detectors and contrast detection (DIC). The Nikon confocal system is ideally suited for imaging rapid physiological events and has spectral imaging capabilities. This system is both a confocal and a widefield system using a highly sensitive EMCCD Hamamatsu camera. An atmospheric control chamber is available for maintaining temperature and CO2 levels. The automated stage provides X and Y control for imaging multiple positions within the culture chamber. Rapid Z-stack acquisition is accomplished with the piezo stage insert to provide the investigator with 3 dimensional images over time (4D imaging).

Light Microscopes available at the MSR provide high quality, light level digital images to OSUCCC researchers. The widefield microscope is equipped with a QICLICK-F-M-12 CCD camera (QImaging) to provide bright field, dark field, phase contrast, and fluorescence capabilities. A CRI Nuance multispectral imaging camera is available for use at the MSR in conjunction with the Zeiss Axioskop microscope to assist the researcher in separating signals in samples prepared using either chromogens or fluorophores. Both cameras can be used with the Olympus SZH Zoom Stereo Microscope system for low magnification imaging of whole mount or large samples not suitable for the widefield system. The stereomicroscope has an auxiliary fluorescence light source, the Nightsea viewing system, capable of illuminating samples containing green fluorescence markers (i.e. GFP).

The Leica CM1510S Cryostat enables the researcher to obtain 5-40 micron tissue sections for examination using light microscopy.

Fees

Listed prices are for OSU or federally-funded investigators.  Please contact SR for external rates.

 

Instrument or Service

Rate

Basis

 

Transmission Electron Microscopy

Transmission Electron Microscope

$35.00

Hour

Transmission Electron Microscopy Sample Preparation Service - Tissue

$100.00

Per 3 Samples

Transmission Electron Microscopy Sample Preparation Service- Cell Monolayer

$110.00

Per 3 Samples

Transmission Electron Microscopy Sample Preparation Service - Cell Pellet

$120.00

Per 3 Samples

TEM Sample Sectioning Service

$80.00

Per Sample

TEM Sample Sectioning Service (Thick sections only per block)

$40.00

Per Sample

 

Scanning Electron Microscopy

Scanning Electron Microscope

$35.00

Hour

Scanning Electron Microscopy Sample Preparation Service

$90.00

Per 3 Samples

Critical Point Dryer

$25.00

Hour

Sputter Coater

$10.00

Hour

 

Confocal Microscopy

Confocal Laser Scanning Microscope (9-5)

$30.00

Hour

Confocal Laser Scanning Microscope after-hours use

$25.00

Hour

 

Multiphoton Microscopy

Multiphoton Confocal Laser Scanning Microscope

$35.00

Hour

Multiphoton Confocal Laser Scanning Microscope DAILY RATE

$280.00

24 Hours

 

Live Cell Microscopy

Live Cell Confocal Laser Scanning Microscope

$30.00

Hour

Live Cell Confocal Laser Scanning Microscope DAILY RATE

$240.00

24 Hours

 

Light Microscopy

Light Microscope - Widefield, Brightfield and Stereo

$10.00

Hour

Spectral Camera (Nuance)

$5.00

Hour

 

Sample Preparation Equipment

Ultramicrotome

$10.00

Hour

Cryo-Ultramicrotome

$30.00

Hour

Cryostat

$10.00

Hour

Vibratome

$10.00

Hour

Microwave Processing

$5.00

Hour

 

Technical Assistance*

$35.00

Hour

* Instrument training and requested user support during self-operation. All services include technical assistance charges.

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