The Microscopy Shared Resource (MSR) offers training tailored to the needs of the individual investigator on all microscope systems in the facility. Unique to the facility, consultation and support on subjects ranging from experimental design, troubleshooting and data analysis are also offered. Processing and analysis software for image presentation and publication is also available to Ohio State cancer researchers.
The MSR is the only facility on campus that provides processing of biological samples for evaluation using electron microscopy. These services include sample processing of TEM and SEM samples requiring different treatments in order to obtain optimal results. For SEM sample processing, the researcher can choose between chemical dehydration or critical point drying depending on the application and desired result. Traditional epoxy resin processing and sectioning are available for TEM sample observation. However, examination of soft materials such as biopolymers used as substrates for cell growth is very difficult to section using this approach. For optimal imaging of soft samples, a cryo-ultramicrotome is available to section these samples after freezing. Cryo-ultramicrotomy is also offered for observation of samples using light microscopy to assist in eliminating out of focus light, an issue that is often a result of autofluorescence. The MSR also offers negative staining as a means of observing samples when dehydration is not suitable for maintaining structure.
Listed prices are for The Ohio State University or federally-funded investigators. Please contact SR for external rates.
Instrument or Service
|Transmission Electron Microscopy|
|Transmission Electron Microscope||$20.00||1/2 Hour|
|Transmission Electron Microscopy Sample Preparation Service - Tissue||$100.00||Per 3 Samples|
|Transmission Electron Microscopy Sample Preparation Service - Cell Monolayer||$110.00/hour||Per 3 Samples|
|Transmission Electron Microscopy Sample Preparation Service - Cell Pellet||$120.00||Per 3 Samples|
|TEM Sample Sectioning Service||$80.00||Per Sample|
|TEM Sample Sectioning Service (Thick sections only per block)||$40.00||Per Sample|
|Scanning Electron Microscopy
|Scanning Electron Microscope||Coming Soon
|Scanning Electron Microscopy Sample Preparation Service||$90.00||Per 3 Samples|
|Critical Point Dryer||$25.00||Hour|
|Cryo-Sample Preparation Service - High Pressure Freezing||$75.00||Per 3 Samples|
|Confocal Laser Scanning Microscope (9-5)||$30.00||Hour|
|Confocal Laser Scanning Microscope after-hours use||$25.00||Hour|
|Multiphoton Confocal Laser Scanning Microscope||$35.00||Hour|
|Multiphoton Confocal Laser Scanning Microscope||$280.00||24 Hours|
|Live Cell Microscopy
|Live Cell Confocal Laser Scanning Microscope||$30.00||Hour|
|Live Cell Confocal Laser Scanning Microscope||$240.00||24 Hours|
|Light Microscope - Widefield, Brightfield and Stereo||$10.00||Hour|
|Spectral Camera (Nuance)||$5.00||Hour|
|Sample Preparation Equipment
|* Instrument training and requested user support during self-operation. All services include technical assistance charges.