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- Targeted and Quantitative sample analysis of nutrients, phytochemicals and their metabolites by HPLC or UHPLC-MS/MS including:
Mass spectrometry is a desirable detector in analytical chemistry due to its powerful combination of sensitivity and selectivity, which is owing to the often unambiguous characteristic of mass:charge ratio (m/z) of molecular ions and patterns of fragmentation during MS/MS experiments. Primary NPASR equipment includes four ultra-high pressure liquid chromatography/photodiode array/tandem mass spectrometry systems:
UHPLC/SelexION/QTrap 5500 (Sciex): a hybrid quadrupole ion trap system which typically operates as a triple quadrupole MS but can perform MS/MS/MS (MS3) experiments for enhanced identification and selectivity. The QTrap is also outfitted with the Sciex SelexION differential ion mobility spectrometry device, providing another dimension of separation which enables the Lipidyzer platform, and other applications.
UHPLC/SFC/Q-TOF 6550 (Agilent): a quadrupole time-of-flight ion funnel instrument for high throughput metabolomics, as well as discovery lipidomics. This system delivers high mass resolution (up to 40,000) and accurate mass capabilities (1-2 ppm) for identification, as well as best in-class sensitivity and four orders of dynamic range. Super-critical fluid chromatography (SFC) allows for separation of neutral and polar lipids in a single injection and since SFC behaves like normal phase chromatography it makes more routine the analysis of very polar compounds that are difficult to retain in reversed phase separations.
UHPLC/Q-TOF 6550 (Agilent): a quadrupole time-of-flight ion funnel instrument which delivers high mass resolution (up to 40,000) and accurate mass capabilities (1-2 ppm) for identification, as well as best in-class sensitivity and four orders of dynamic range. It is used primarily for untargeted metabolomics experiments and identification work.
UHPLC/QqQ 6495 (Agilent): a triple quadrupole with ion funnel optics provides the utmost sensitivity and dynamic range of our instruments allowing for outstanding quantitation. HPLC (Waters): 2 Alliance 2695 systems with 996 and 2996 photodiode arrays (PDA) to quantify small molecules with suitable chromophores. PDA detectors are in-line on all LCMS systems as well to collect UV-vis and MS spectra.