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Services

The Small Animal Imaging Core (SAIC) is a state-of-the-art, small animal imaging facility available to investigators at OSUCCC – James as well as to other academic and commercial institutions.

Services include:

High-Resolution Image Data Acquisition

The SAIC provides high-resolution anatomical and functional imaging of rodent models of disease and ex vivo specimens. Technicians and imaging scientists trained in the use of the specialized image acquisition equipment will collect high-quality image data for individual investigators. In-house training of an investigator’s laboratory personnel in use of the imaging equipment (ultrasound and BLI/FL) is also available to those groups who demonstrate a specific need for extensive imaging studies.

Data Interpretation

The SAIC experts help investigators interpret images obtained from the facility’s instrumentation.Our associates are experienced in distinguishing normal and abnormal features in in vivo images of animals, and have the training and background to differentiate imaging artifacts from real results. Investigators are encouraged to review their results and data interpretation with SAIC personnel upon imaging study completion.

Image Post-Processing and Quantitative Image Analysis

The SAIC offers quantitative image analysis, and each imaging instrument has modality-dependent image analysis software. Additional image analysis and data interpretation is provided via open-source image analysis software and custom-designed software developed by the SAIC and OSC.

Consultation on Design Imaging Studies

The SAIC’s imaging scientists can help design imaging studies for each investigator’s specific research applications. We recommend contacting a SAISR representative prior to setting up an imaging study to determine the most appropriate imaging modality and protocol for each imaging study.

Training and Education

The SAIC provides hands-on training for individual imaging modalities and workshops on image acquisition and data analysis. We also hold seminars on specific research topics in small animal imaging.

Instrumentation Equipment

The Small Animal Imaging Core (SAIC) facility houses high-resolution small animal imaging instrumentation and offers assistance from experts in: operating each imaging modality, small animal handling procedures and analytical software support for quantitative image analysis.

The following micro-imaging equipment includes magnetic resonance imaging (MRI), high-frequency ultrasound (US), bioluminescence and fluorescence optical Imaging system (BLI/FL), echoMRI for body composition analysis, and X-ray irradiation platform (SARRP). 

All imaging instrumentation comes fully equipped with biological (ECG, respiration, temperature) monitoring equipment and isoflurane anesthesia systems. The instrumentation can be used for in vivo animal imaging or ex vivo specimen investigations. 

High-Field Magnetic Resonance Imaging (MRI)

The BioSpec 94/30 Imaging System is a 9.4T horizontal bore magnet that operates at 400 MHz and runs ParaVision™ 5.1 software. This microMRI system is designed for high-resolution MR spectroscopy and imaging of in vivo or ex vivo specimens.

The 30 cm diameter bore and extra-long table offers imaging capabilities of 1H, 13C, 19F, 23 Na, 31P on mice, rats, small rabbits and ex vivo specimens. The BioSpec also includes a BioTrig monitoring unit and in vivo cardiac gating.

MRI applications include:

  • Skeletal and soft tissue abnormalities
  • DCEMRI for tumor/tissue vascularization and permeability
  • In vivo spectroscopy to measure metabolites (e.g. 31P)
  • In vivo localized spectroscopy to measure metabolites (1H)
  • Cardiac imaging for assessing cardiac function, ischemia and infarcts
  • T1, T2, and Diffusion maps

The Vevo 2100 High-Frequency Ultrasound System is capable of 2D and 3D in vivo imaging in small animals and has a 30-micron spatial resolution and frame rates of up to 1000 fps. The system includes an 18, 24, 38 and 55 MHz transducer, a needle-guided injection apparatus, and is capable of color, pulsed wave and power Doppler, 3D imaging and advanced image analysis.

High-Frequency Ultrasound (US)

The Vevo 2100 High-Frequency Ultrasound System is capable of 2D and 3D in vivo imaging in small animals and has a 30-micron spatial resolution and frame rates of up to 1000 fps. The system includes an 18, 24, 38 and 55 MHz transducer, a needle-guided injection apparatus, and is capable of color, pulsed wave and power Doppler, 3D imaging and advanced image analysis.

The Vevo can be used for:

  • Image-guided injections
  • Evaluating developmental biology – mouse and rat embryos
  • Calculating tumor size and blood flow
  • Quantitative evaluation of cardiac physiological function and development

Optical Imaging System (BLI and FL)

A CaliperTM IVIS Lumina II Bioluminescent Optical Imaging System is suitable for 2D in vivo and ex vivo bioluminescence and fluorescence imaging. The system is equipped with four standard emission filters ICG, Cy5.5, DsRed, GFP (810-875, 695-770, 575-650, 515-575 nm) for fluorescence imaging.

IVIS Lumina II applications include:

  • A powerful labeling technique through gene expression results in production of luciferase
  • Provides analysis tools for quantification
  • Provides in vivo fluorescence imaging (preferable near-infrared)
  • Provides ex vivo and in vitro BLI and FL imaging

CT and Therapeutic X-ray (SARRP)

The SARRP system (XSTRAHL) delivers clinically relevant radiation protocols under preclinical conditions. The SARRP system can deliver radiation dose locally with very precise collimators from 0.5 mm small focal spot to 10*10 mm window, variable collimators up to 40*80 mm, or with open field (typical 18*18 cm, bigger possible)

The SARRP system can be used for:

  • local tumor treatment with minimalized dose to whole body or individual organs
  • to sensitize lesion to different treatment
  • Total Body Irradiation (TBI) for e.g. bone marrow or autologous stem cell transplantation
  • to study skin wound etc.

Body Composition analyzer

EchoMRI Analyzer delivers precise body composition measurements of fat, lean, free water, and total water masses in live animals, organ or biopsy. Scanning takes 0.5 – 3.2 minutes. The live animals need no anesthesia and no special preparation before measurement.

Fees FY2017

Service/Supply

Ohio State users or
federally funded
investigators

Non-Ohio State User

Bioluminescene and Fluorescence (IVIS Lumina II) - user operated $69.00 Hour $125.00 Hour
Bioluminescene and Fluorescence (IVIS Lumina II) - core assisted $114.00 Hour $170.00 Hour
IVIS Training $75.00 Hour $120.00 Hour
Ultrasound (Vevo 2100) - user operated $69.00 Hour $130.00 Hour
Ultrasound (Vevo 2100) - core assisted $114.00 Hour $175.00 Hour
Vevo 2100 Training $75.00 Hour $120.00 Hour
9.4 T MRI $148.50 Hour $250.00 Hour
EchoMRI - user operated
$40.00 Hour $40.00 Hour
 EchoMRI - core operated
 $85.00 Hour
$85.00 Hour
Data Analysis $75.00 Hour $75.00 Hour
Xstrahl - Radiation Platform $110.00 Hour
$175.00 Hour
Contrast Agent Market Price Market Price
Luciferase Vial / Market Price Vial / Market Price
Data key replacement $5,000.00 $5,000.00

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