The Small Animal Imaging Core (SAIC) is a state-of-the-art, small animal imaging facility available to investigators at OSUCCC – James as well as to other academic and commercial institutions.
High-Resolution Image Data Acquisition
The SAIC provides high-resolution anatomical and functional imaging of rodent models of disease and ex vivo specimens. Technicians and imaging scientists trained in the use of the specialized image acquisition equipment will collect high-quality image data for individual investigators. In-house training of an investigator’s laboratory personnel in the use of the imaging equipment is also available.
The SAIC experts help investigators interpret images obtained from the facility’s instrumentation.Our associates are experienced in distinguishing normal and abnormal features in in vivo images of animals and have the training and background to differentiate imaging artifacts from real results. Investigators are encouraged to review their results and data interpretation with SAIC personnel upon imaging study completion.
Image Post-Processing and Quantitative Image Analysis
The SAIC offers quantitative image analysis, and each imaging instrument has modality-dependent image analysis software. Additional image analysis and data interpretation is provided via open-source image analysis software and custom-designed software developed by the SAIC and OSC. SAIC also provides access (in the lab or remotely) to workstation PCs for data analysis.
Consultation on Design Imaging Studies
The SAIC’s imaging scientists can help design imaging studies for each investigator’s specific research applications. We recommend contacting a SAIC representative prior to setting up an imaging study to determine the most appropriate imaging modality and protocol for each imaging study.
Training and Education
The SAIC provides hands-on training for individual imaging modalities and workshops on image acquisition and data analysis. We also hold seminars on specific research topics in small animal imaging.
The Small Animal Imaging Core (SAIC) facility houses high-resolution small animal imaging instrumentation and offers assistance from experts in: operating each imaging modality, small animal handling procedures and analytical software support for quantitative image analysis.
The following micro-imaging equipment includes magnetic resonance imaging (MRI), high-frequency ultrasound (US), bioluminescence and fluorescence optical imaging system (BLI/FL), echoMRI for body composition analysis, micro-Computed Tomography (microCT) and X-ray irradiation platform (SARRP).
All imaging instrumentation comes fully equipped with biological (ECG, respiration, temperature) monitoring equipment and isoflurane anesthesia systems. The instrumentation can be used for in vivo animal imaging or ex vivo specimen investigations.
High-Field Magnetic Resonance Imaging (MRI)
The BioSpec 94/30USR (Bruker BioSpin Co.) Imaging System is a 9.4T horizontal bore magnet that operates at 400 MHz and runs ParaVision™ 6.0 software. This microMRI system is designed for high-resolution MR spectroscopy and imaging of in vivo or ex vivo specimens.
The 30 cm diameter bore and extra-long table offers imaging capabilities of 1H, 13C, 19F, 23Na, 31P on mice, rats, small rabbits and ex vivo specimens. The BioSpec also includes a BioTrig monitoring unit and in vivo cardiac gating (SAII and IntraGate).
MRI applications include:
- Skeletal and soft tissue abnormalities
- DCE-MRI for tumor/tissue vascularization and permeability
- spectroscopy to measure metabolites (e.g. 31P)
- localized spectroscopy to measure metabolites (1H)
- Cardiac imaging for assessing cardiac function, ischemia and infarcts (Cardio Package)
- T1, T2 and Diffusion maps
- Diffusion Tensor Imaging
Computed Tomography (micro-CT)
The SkyScan 1276 (Bruker BioSpin Co.) is a high-performance, standalone, fast, desktop in vivo microCT with continuously variable magnification for scanning small laboratory animals (mice and rats) and biological samples. It has an unrivalled combination of high resolution (2.8µm), big image size as well as software allowing to acquire data with breathing and ECG gating. It is capable of reconstructing 3D high resolution images throughout the animal body that can be used for quantitative morphometric measurements. The software also allows to perform quantitative bone density measurement.
The SkyScan system can be used for:
- Whole body mouse imaging
- Lung imaging in mice and rats (e.g. tumor monitoring)
- Bone imaging and bone density measurements
- Cardiovascular imaging
- Vascularization imaging e.g. brain, tumors, ischemic tissue
- Post contrast imaging for miscellaneous applications
CT and Therapeutic X-ray (SARRP)
The SARRP system (XSTRAHL Inc.) delivers clinically relevant radiation protocols under preclinical conditions. The SARRP system can deliver radiation dose locally with very precise collimators from 0.5 mm small focal spot to 10*10 mm window, variable collimators up to 40*80 mm or with open field (typical 15*15 cm, bigger field is possible per application)
The SARRP system can be used for:
- Local tumor treatment with minimalized dose to whole body or individual organs
- To sensitize lesion to different treatment
- Total Body Irradiation (TBI) for e.g. bone marrow or autologous stem cell transplantation
- Studying skin wounds
High-Frequency Ultrasound (US)
The Vevo 2100 (FujiFilm Co.) High-Frequency Ultrasound System is capable of 2D and 3D in vivo imaging in small animals and has a 30-micron spatial resolution and frame rates of up to 1000 fps. The system includes an 18, 24, 38 and 55 MHz transducer, a needle-guided injection apparatus and is capable of color, pulsed wave and power Doppler, 3D imaging and advanced image analysis.
The Vevo can be used for:
- Image-guided injections
- Evaluating developmental biology – mouse and rat embryos
- 3D-Mode Imaging and Volume Analysis (e.g. tumor size)
- Contrast imaging e.g. for tissue or tumor blood perfusion
- Quantitative evaluation of cardiac physiological function and development
- Power and Color Doppler modes for blood flow quantification
Optical Imaging System (BLI and FL)
An IVIS Lumina II (PerkinElmer Inc.) Bioluminescent Optical Imaging System is suitable for 2D in vivo and ex vivo bioluminescence and fluorescence imaging. The system is equipped with four standard emission filters ICG, Cy5.5, DsRed, GFP (810-875, 695-770, 575-650, 515-575 nm) for fluorescence imaging.
IVIS Lumina II applications include:
- Imaging of a powerful labeling technique through gene expression results in production of luciferase
- Provides in vivo fluorescence imaging (preferable near-infrared)
- Provides ex vivo and in vitro BLI and FL imaging
- Provides analysis tools for quantification
Body Composition Analyzer
EchoMRI (EchoMRI LLC) Analyzer delivers precise body composition measurements of fat, lean, free water and total water masses in live animals (up to 100g), organ or biopsy (up to 300µg). Scanning takes 0.5-3.2 minutes. The live animals need no anesthesia and no special preparation before measurement.
EchoMRI system can be used for cancer, cardiac, toxicology, diabetic, nutritional studies and others
Non-Ohio State user
|Bioluminescene and Fluorescence (IVIS Lumina II) - User Operated||$69.00/hour||$130.00/hour|
|Bioluminescene and Fluorescence (IVIS Lumina II) - Core Assisted||$114.00/hour||$175.00/hour|
|Bioluminescene and Fluorescence (IVIS Lumina II) -Training||$75.00/hour||$120.00/hour|
|echoMRI - User Operated||$40.00/hour||$100.00/hour|
|echoMRI - Core Assisted||$85.00/hour||$145.00/hour|
|echoMRI - Training||$75.00/hour||$120.00/hour|
|Ultrasound (Vevo 2100) - User Operated||$70.00/hour||$130.00/hour|
|Ultrasound (Vevo 2100) - Core Assisted||$115.00/hour||$175.00/hour|
|Ultrasound (Vevo 2100) - Training||$75.00/hour||$120.00/hour|
|9.4 T MRI - Core Operated||$150.00/hour||$250.00/hour|
|9.4 T MRI - Long Run||$850.00/specimen||TBD|
|9.4 T MRI - Short Run||$600.00/specimen||TBD|
|Radiation Platform (SARRP) - User Operated||$55.00/hour||$130.00/hour|
|Radiation Platform (SARRP) - Core Operated||$55.00/hour||$175.00/hour|
|Radiation Platform (SARRP) -Cells Irradiation - User Operated||$50.00/hour||$125.00/hour|
|Radiation Platform (SARRP) - Cells Irradiation - Core Operated||$50.00/hour||$170.00/hour|
|Radiation Platform (SARRP) - Training||$75.00/hour||$120.00/hour|
|microCT (SkyScan 1276) - User Operated||$75.00/hour||$135.00/hour|
|microCT (SkyScan 1276) - User Operated 1st Year of Operation||$55.00/hour||$135.00/hour|
|microCT (SkyScan 1276) - Core Operated||$120.00/hour||$180.00/hour|
|microCT (SkyScan 1276) - Core Operated 1st Year of Operation||$100.00/hour||$180.00/hour|
|microCT (SkyScan 1276) - Training||$75.00/hour||$120.00/hour|
|Data Key Replacement Fee||$5,000.00 each||$5,000.00 each|
|Contrast Agents||Market Price||Market Price|
|Special Supplies||Market Price||Market Price|
Prices are effective: July 1st, 2019. Contact core manager for any questions regarding service rates.
*Business hours are 8:00 a.m.-5:00 p.m, Monday-Friday. University holidays excluded.