Director: Dario Palmieri, PhD
Technical Director: Lara Rizzotto, PhD
Gene editing using CRISPR/Cas technologies is one of the most advanced, cutting-edge approaches to study gene, RNA and protein functions by targeting the endogenous genetic code of the cells of interest. Furthermore, it can be used to design and validate novel diagnostic and therapeutic tools for a wide range of human diseases, including cancer.
The GESR provides tailored services using CRIPSR-based technologies to support the needs of all its users. Services include (but are not limited to):
- Gene-editing services using immortalized cell lines and primary cells
- Design of CRISPR knock-out and knock-in approaches
- gRNA cloning and/or delivery as RNP complexes
- Design of repair templates for small knock-ins and point mutations
- Generation of vectors and cell lines expressing biosensors for CRISPR-based screens
- Design and generation of large repair templates
- Generation of CRISPR-KO/KI bulk cell lines or single clones
- Cell sorting and live-cell imaging of gene-edited cells
- Cas13-based RNA targeting
- Approaches for gene editing without DNA double-strand breaks (base editing and PRIME edit)
- Design and generation of libraries for CRISPR screenings
- CRISPR-based genetic screenings
GESR provides the investigators with cutting-edge gene editing technologies, thoroughly participating in the 1) study design; 2) identification of optimal strategies; 3) generation of preliminary data to assess project feasibility; and 4) generation of gene-edited primary and established cell lines.
GESR can perform DNA-based (using recombinant plasmids) and DNA-free (using synthetic Cas9/RNP complexes) gene editing projects. In addition GESR is provided with fully equipped lab space for the preparation of reagents required for gene editing projects, as well as tissue culture rooms for the generation of cell lines required by the investigators.
Resources dedicated to the GESR include:
- Dedicated lab space for the production of reagents required for gene-editing projects (e.g., molecular cloning and cell genotyping)
- Dedicated tissue culture room, including all the standard equipment required for manipulation of primary and established cell lines (2 biosafety cabinets, 2 centrifuges and 1 light microscope)
- Lonza 4D Core Unit system equipped for optimal high-throughput electroporation (Lonza 96-well Shuttle)
- EVOS M5000 microscope equipped with multiple light cubes for bright-field and fluorescence microscopy, magnification objectives ranging from 4X to 100X and onstage incubator for live-cell imaging
- CountessFL automated cell counter
- Sony MA900 cell sorter, equipped with four lasers and 12 detectors, capable of simultaneous sorting of up to four individual cell populations and single-cell plating. The chip-based system allows the selection of appropriate pressure conditions to maximize purity, yield and cell viability in the sorting process.
Contact GESR
Ohio State University Comprehensive Cancer Center
994 Biomedical Research Tower
460 West 12th Avenue
Columbus, OH 43210
614-366-2551
Dario.Palmieri@osumc.edu
| Service | Unit | FY25 Internal Rate | FY25 External Rate | FY25 Industry Rate |
| Sorting Time | Day | $720.00 | $1,134.000 | $1,211.04 |
| Labor | Hour | $240.00 | $378.000 | $403.68 |