Expertise
- HTBSR Director: Lapo Alinari, MD, PhD
- HTBSR Assistant Director: Christopher Manring, MBA
- HTBSR Program Coordinator: Lori Del Riesgo
Lapo Alinari, MD, PhD, is a tenured Associate Professor of Internal Medicine with a clinical and research focus in B-cell Lymphoma. He was appointed as the OSUCCC Hematology Tissue Bank Shared Resource Director (HTBSR) in the summer of 2019 so this facility could better align its services to the needs of investigators. Under his leadership, the HTBSR expanded its services to collect samples from patients with any sort of hematologic disorders, umbilical cord blood samples as a source of hematopoietic stem cells, and discarded normal hipbone as a source of bone marrow stromal cells. The HTBSR received an “exceptional” score at the most recent CCSG renewal and was awarded College of American Pathologist accreditation in 2023 under his leadership.
Christopher Manring, MBA, the HTBSR assistant director, has nearly 13 years of biorepository and laboratory experience, including 16 with the OSUCCC HTB. His expertise includes research protocol implementation, sample processing, data and project management, as well as quantitative analysis and leadership. Additionally, Chris provides clinical trial finance expertise obtained through previous positions collaborating with industry and federal sponsors on clinical trial financial (budget) and regulatory implementation.
Equipment
The HTBSR operates in accordance with NCR Best Practices for sponsored repositories and offers OSUCCC members and the cancer research community access to the following instrumentation, equipment and supplies:
Revvity Ascend Cellometer for enumerating cell counts and viability data via bright field imaging and dual-fluorescence imaging to increase accuracy and consistency.
Benchtop Centrifuges for mononuclear cell separation.
Biological safety Cabinets for sterile processing (and, if needed, cell culture) of samples.
Mouthwash Collection Kits for the collection and extraction of normal DNA.
Computer Workstations for electronic database management.
Dangerous Goods Shipping Containers for sending samples to approved outside investigators.
Dry Ice for on-campus transport or shipping.
MVE Automated Filling System for efficient liquid nitrogen (LN2) use. The system includes a service area and automatic tank switcher where up to four LN2 tanks can be stationed. Vacuum-jacketed piping leads to filling stations for each LN2 freezer.
MVE LN2 Freezers for safe long-term sample storage without direct contact with LN2. Freezers hold 15,500-36,400 samples, depending on rack size and storage box profile.
NanoDrop Spectrophotometer for spectrophotometric analysis of absorbance ratios to quantify derivative specimens. This spectrophotometer quickly determines the concentration of RNA, DNA or protein, and uses an extremely small sample volume. This enables the HTBSR to perform appropriate quality checks of samples while preserving the bulk of the sample for research.
Discovery Echo Microscope for counting blasts in peripheral blood and bone marrow aspirate specimens as well as fibroblast observation/counting for cell culture of skin punch biopsies.
Centrak Environmental Monitoring System for 24/7 monitoring of all HTBSR freezers and refrigerators. The Cenrak system provides a real time readout of monitored units and immediate telecommunication with individuals in the event of temperature fluctuation beyond the specified range.
Refrigerated Microcentrifuges for derivative sample preparation.
Eppendorf CO2 Incubator for cell culture growth
Qubit Fluorometer for highly sensitive determination of the concentration and quality of derivative specimens while preserving the bulk of the sample for research.