The PSR provides OSUCCC – James members with complete proteomic support using state-of-the-art electrophoresis and imaging equipment, robotic sample handlers and mass spectrometers. The PSR offers extensive expertise in identifying proteins from protein complexes utilizing leading mass spectrometer instrumentation and supporting equipment for a wide range of proteomic analysis, protein identification and post-translational modifications identification.
The PSR’s instruments include:
Bruker 15 T SolariX FT-ICR: Offers ultra-high-resolution/mass accuracy as well as the isotopic footprint of small molecules. The instrument has a dual source (MALDI and ESI) that can be used for proteomics/metabolomics investigation, ion-molecule reactions and MALDI imaging.
Bruker ultrafleXtreme MALDI-TOF/TOF equipped with Proteineer fc II for LC-MALDI and ImagePrep for Tissue imaging sample preparation: 2 KHz speed TOF and smartbeam-II laser for ultra-high data acquisition in both MS and MS/MS mode. Currently, this instrument is mainly used for protein profile analysis, peptide mass mapping and synthetic polymer and lipid analyses.
Bruker timsTOF Pro: This platform combines nano-LC separation (liquid phase) with ion mobility separation (gas phase) to enhance the separation of peptides generated by digestion of complex protein mixture. By using these two “orthogonal” separation methods we can reliably identify large numbers of proteins (>3,000) with shorter analysis time (compared to 2D LC measurements).
Thermo Orbitrap Eclipse: Ultra-high resolution mass spectrometer with extended mass range up to m/z 8000 coupled with a nano-LC for the analysis of intact proteins and peptides. The instrument is equipped with multiple methods of fragmentation including collision induced dissociation (CID and HCD), and electron transfer dissociation (ETD). The Eclipse is also equipped with proton transfer charge reduction (PTCR) which simplifies complex top-down spectra. The instrument is well suited for advanced proteomics including bottom-up and top-down studies to identify proteins and post-translational modifications. We can perform relative quantitation studies, targeted quantitation studies, and tandem mass tag (TMT) based quantitation on this platform.
Thermo Orbitrap Fusion: Ultra-high-resolution LC-MS equipped with different fragmentation methods (CID, HCD and ETD), the system is also equipped with Dionex nano-LC system for advanced proteomics, including bottom-up proteomics, post-translational modifications, relative quantitation, targeted quantitation and intact protein characterization.The nano-LC can be switched between 1D and 2D LC for to identify more proteins. A FAIMS ion mobility device is under installation and will be used in near future.
Thermo QE Plus Orbitrap: Ultra-high-resolution LC-MS complete with Dionex nano-LC system for advanced proteomics, including bottom-up proteomics, post-translational modifications, relative quantitation, targeted quantitation and intact protein characterization. The nano-LC can be switched between 1D and 2D LC to benefit the identification of proteins from more complex system.
Thermo QE Plus Orbitrap: This is a recently acquired and installed ultra-high-resolution instrument with micro-HPLC capability. It will be used mostly (but not exclusively) for metabolomics studies.
Thermo Exploris 480: Ultra-high resolution mass spectrometer coupled with a Vanquish HPLC for metabolomics and proteomics analysis. This platform is used for targeted and untargeted studies and has resolution up to 480, 000. The Exploris 480 can achieve sub-ppm accuracy and with this capability has enough resolution to reveal the chemical formula of even unknown metabolites with a much higher degree of certainty than previous instruments. The 480, due to its high dynamic range and sensitivity, can provide high quality linear calibration curves without the need for time-consuming optimization of specific targets. Additionally, the instrument utilizes AcquireX which, can direct the data-dependent and data-independent scanning of small molecule metabolomics investigations that will allow the instrument to reduce the time spent fragmenting background ions and create libraries of compounds identified from untargeted experiments to focus future experiments with targeted analysis
Thermo Quantiva: A triple quadrupole mass spectrometer with ultrahigh sensitivity equipped with an HPLC sysrtem for quantitative (targeted) analysis of metabolites and peptides.
Matrix Science Mascot Server: A powerful search engine for mass spectrometry data to identify proteins and peptide modifications.
Proteome Software Scaffold 4.9.0: Used to visualize complex proteomic data and to calculate spectral counting for label-free experiments.
ProteomeDiscoverer 2.2:: Powerful platform that can be used to search the data by MASCOT and sequest for protein identification as well as protein quantitation using TMT or SILAC.